Protecting monoclonal antibodies via competitive interfacial adsorption of nonionic surfactants

IF 9.7 1区 化学 Q1 CHEMISTRY, PHYSICAL
Zeyuan Zhuang , Haoran Wu , Zongyi Li , Mingrui Liao , Kangcheng Shen , Renzhi Li , Stephen Hall , Cavan Kalonia , Kai Tao , Xuzhi Hu , Jian Ren Lu
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Abstract

Hypothesis

Bioengineered monoclonal antibodies (mAbs) have gained significant recognition as medical therapies. However, during processing, storage and use, mAbs are susceptible to interfacial adsorption and desorption, leading to structural deformation and aggregation, and undermining their bioactivity. To suppress antibody surface adsorption, nonionic surfactants are commonly used in formulation. But how surface hydrophobicity affects the adsorption and desorption of mAbs and nonionic surfactants individually and as a mixture remains inconclusive.

Experiments

The rapid tuning of the siliconized surface from hydrophobic to hydrophilic was controlled by the UV oxidation time of a self-assembled trimethoxy(7-octen-1-yl)silane (TMOS) monolayer. Spectroscopic ellipsometry and neutron reflection were used to determine the dynamic adsorption and structural changes of the co-adsorbed mAb (COE-3) and the commercial nonionic surfactant PS80, which is composed primarily of polyoxyethylene-sorbitan monooleate with an average molecular weight of about 1310 g/mol.

Findings

COE-3 adsorption on both TMOS or UV-TMOS surface was irreversible. However, nonionic surfactant PS80 could partially remove pre-adsorbed COE-3 from these surfaces, forming a co-adsorption layer. Interestingly, while the hydrophobic TMOS surface prevented mAb adsorption when pre-treated with PS80, the amphiphilic UV-TMOS did not. Furthermore, when COE-3 and PS80 were injected as a mixture, PS80 formed a preventative layer on both surfaces against COE-3 adsorption. These results highlight the significance of surface hydrophobicity in controlling mAb adsorption in the presence of nonionic surfactants.

Abstract Image

通过非离子表面活性剂的竞争性界面吸附保护单克隆抗体。
假设:生物工程单克隆抗体(mab)作为一种医学疗法已经获得了广泛的认可。然而,在制备、储存和使用过程中,单克隆抗体容易受到界面吸附和解吸,导致结构变形和聚集,破坏其生物活性。为了抑制抗体的表面吸附,配方中通常使用非离子型表面活性剂。但表面疏水性如何影响单克隆抗体和非离子表面活性剂的单独和混合吸附和解吸仍然没有定论。实验:通过紫外氧化时间控制自组装三甲氧基(7-辛烯-1-基)硅烷(TMOS)单分子膜由疏水到亲水性的快速调整。利用椭偏光谱和中子反射光谱研究了共吸附mAb (COE-3)和商品非离子表面活性剂PS80的动态吸附和结构变化。PS80主要由聚氧乙烯-山梨醇单油酸酯组成,平均分子量约为1310 g/mol。结果:COE-3在TMOS和UV-TMOS表面的吸附都是不可逆的。而非离子表面活性剂PS80可以部分去除预吸附的COE-3,形成共吸附层。有趣的是,当用PS80预处理时,疏水性TMOS表面阻止单抗吸附,而两亲性UV-TMOS则没有。此外,当COE-3和PS80作为混合物注入时,PS80在两表面形成防止COE-3吸附的保护层。这些结果突出了表面疏水性在非离子表面活性剂存在下控制单抗吸附的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
16.10
自引率
7.10%
发文量
2568
审稿时长
2 months
期刊介绍: The Journal of Colloid and Interface Science publishes original research findings on the fundamental principles of colloid and interface science, as well as innovative applications in various fields. The criteria for publication include impact, quality, novelty, and originality. Emphasis: The journal emphasizes fundamental scientific innovation within the following categories: A.Colloidal Materials and Nanomaterials B.Soft Colloidal and Self-Assembly Systems C.Adsorption, Catalysis, and Electrochemistry D.Interfacial Processes, Capillarity, and Wetting E.Biomaterials and Nanomedicine F.Energy Conversion and Storage, and Environmental Technologies
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