Physical and Chemical Analysis of the Lipofuscin Granule of Bisretinoid Photodestruction Products From Retinal Pigment Epithelium Cells of the Eye

IF 1.4 4区化学 Q4 PHYSICS, ATOMIC, MOLECULAR & CHEMICAL

Russian Journal of Physical Chemistry B Pub Date : 2025-01-15 DOI:10.1134/S1990793124701276

M. A. Yakovleva, A. A. Vasin, A. E. Dontsov, A. A. Gulin, A. V. Aybush, A. A. Astafiev, A. M. Shakhov, T. B. Feldman, M. A. Ostrovsky

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Abstract

In this paper, the mechanisms of the formation of the bisretinoid oxidation products in lipofuscin granules (LGs) isolated from the retinal pigment epithelium cells of the human eye are studied. The physicochemical characteristics of the bisretinoid photooxidation products are described. The methods of IR spectroscopy, Raman spectroscopy, fluorescence spectroscopy, scanning confocal microscopy, time-of-flight mass spectrometry of secondary ions, and high-performance liquid chromatography are used for the study. The properties of the products of photooxidation and degradation of the fluorophore of LGs, including synthetic N-retinylidene-N-retinylethanolamine (A2E), are described in detail. It is shown that the products of oxidative degradation of LGs are similar to the products of photooxidation of the main bisretinoid of LGs: A2E. These data are important both for understanding the mechanisms of the formation of cytotoxic products in LGs and for establishing their chemical nature.

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眼视网膜色素上皮细胞类双维甲酸光破坏产物脂褐素颗粒的理化分析

本文研究了从人眼视网膜色素上皮细胞分离的脂褐素颗粒（lipofuscin granules, LGs）中类双维甲酸氧化产物的形成机制。介绍了类双维甲酸光氧化产物的理化特性。采用红外光谱、拉曼光谱、荧光光谱、扫描共聚焦显微镜、二次离子飞行时间质谱、高效液相色谱等方法进行研究。详细介绍了LGs荧光团光氧化降解产物的性质，包括合成n -视黄醛- n -视黄醛乙醇胺（A2E）。结果表明，LGs的氧化降解产物与LGs的主要类双维甲酸A2E的光氧化产物相似。这些数据对于理解LGs中细胞毒性产物的形成机制和确定其化学性质都很重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Russian Journal of Physical Chemistry B 化学-物理：原子、分子和化学物理

CiteScore

2.20

自引率

71.40%

发文量

106

审稿时长

4-8 weeks

期刊介绍： Russian Journal of Physical Chemistry B: Focus on Physics is a journal that publishes studies in the following areas: elementary physical and chemical processes; structure of chemical compounds, reactivity, effect of external field and environment on chemical transformations; molecular dynamics and molecular organization; dynamics and kinetics of photoand radiation-induced processes; mechanism of chemical reactions in gas and condensed phases and at interfaces; chain and thermal processes of ignition, combustion and detonation in gases, two-phase and condensed systems; shock waves; new physical methods of examining chemical reactions; and biological processes in chemical physics.