Genome-wide characterization of the TRP gene family and transcriptional expression profiles under different temperatures in gecko Hemiphyllodactylus yunnanensis

IF 2.2 2区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Chao Li , Xiaoying Liu , Chaochao Hu, Jie Yan, Yanfu Qu, Hong Li, Kaiya Zhou, Peng Li
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Abstract

Temperature is closely linked to the life history of organisms, and thus thermoception is an important sensory mechanism. Transient receptor potential (TRP) ion channels are the key mediators of thermal sensation. In this study, we analyzed the sequence characteristics of TRPs in gecko Hemiphyllodactylus yunnanensis and compared the phylogenetic relationships of TRP family members among different Squamata species. In addition, we sequenced the transcriptome of skin and brain tissues of H. yunnanensis exposed to 12 °C (cold), 20 °C (cool), 28 °C (warm), and 36 °C (hot). The results showed that a total of 591 TRPs were identified in the genomes of 21 Squamate species, and these genes were classified into six subfamilies. Among them, 26 TRP genes were identified in H. yunnanensis and distributed on 13 chromosomes. Overall, TRP genes were conserved in squamates. Based on the transcriptome results, we found a total of 9 TRP genes expressed in the brain and skin of H. yunnanensis, of which six TRP genes were under positive selection. TRPP1L2, TRPP1L3, and TRPV1 were involved in heat-sensitive responses (> 36 °C), and TRPV3, TRPA1, and TRPM8 were involved in cold-sensitive responses (< 20 °C). TRPM8 and TRPP1L2 were important cold and heat sensors in H. yunnanensis, respectively.

Abstract Image

云南壁虎TRP基因家族的全基因组特征及不同温度下的转录表达谱
温度与生物体的生命史密切相关,因此,温觉是一种重要的感觉机制。瞬时受体电位(TRP)离子通道是热感觉的关键介质。本研究分析了云南壁虎TRP的序列特征,并比较了不同鳞目壁虎TRP家族成员的系统发育关系。此外,我们对暴露于12°C(冷)、20°C(冷)、28°C(暖)和36°C(热)环境下的云南猿猴皮肤和脑组织的转录组进行了测序。结果表明,在21种鳞动物的基因组中共鉴定出591个TRPs基因,并将这些基因划分为6个亚科。其中,26个TRP基因在云南水蛭中被鉴定,分布在13条染色体上。总的来说,TRP基因在鳞片中是保守的。根据转录组分析结果,我们发现云南猿猴脑和皮肤共有9个TRP基因表达,其中6个TRP基因处于正选择状态。TRPP1L2、TRPP1L3和TRPV1参与热敏反应(bbb36°C), TRPV3、TRPA1和TRPM8参与冷敏反应(< 20°C)。TRPM8和TRPP1L2分别是云南柽柳重要的冷传感器和热传感器。
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来源期刊
CiteScore
5.10
自引率
3.30%
发文量
69
审稿时长
33 days
期刊介绍: Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology. Part D: Genomics and Proteomics (CBPD), focuses on “omics” approaches to physiology, including comparative and functional genomics, metagenomics, transcriptomics, proteomics, metabolomics, and lipidomics. Most studies employ “omics” and/or system biology to test specific hypotheses about molecular and biochemical mechanisms underlying physiological responses to the environment. We encourage papers that address fundamental questions in comparative physiology and biochemistry rather than studies with a focus that is purely technical, methodological or descriptive in nature.
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