Olive Fruit Phenolic Profiling Using High Resolution-Magic Angle Spinning (HR-MAS) Solid-State NMR Spectroscopy.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4334-1_16

Εfstathia Manolopoulou, Apostolos Spyros

引用次数: 0

Abstract

High Resolution-Magic Angle Spinning (HR-MAS) solid-state NMR spectroscopy is finding increasing application in the analysis of solid foods, bypassing the need for complicated solvent extraction procedures. In the present protocol, we report a simple analytical approach based on HR-MAS NMR spectroscopy for the phenolic profiling of olive fruits, flesh, or skin. This approach allows the facile characterization of phenolic compounds in olive fruits cultivated for extra-virgin olive oil production as a function of maturation and variety, in addition to processing technology for table olives.

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利用高分辨率-魔角旋转（HR-MAS）固态核磁共振光谱分析橄榄果酚类物质。

高分辨率-魔角旋转（HR-MAS）固体核磁共振波谱在固体食品分析中的应用越来越多，绕过了复杂的溶剂提取过程的需要。在本协议中，我们报告了一种基于HR-MAS核磁共振光谱的简单分析方法，用于橄榄果实，果肉或皮肤的酚类分析。除了食用橄榄的加工技术外，这种方法还可以简单地表征用于生产特级初榨橄榄油的橄榄果实中酚类化合物的成熟和品种的功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.