Analysis of Urinary Metanephrines Using Liquid Chromatography Tandem Mass Spectrometry.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4334-1_14

Marlene N Thaitumu, Elizabeth L Frank

引用次数: 0

Abstract

Metanephrines (metanephrine [MN] and normetanephrine [NMN]) are O-methylated metabolites derived from the catecholamines, epinephrine, and norepinephrine, respectively. High concentrations of metanephrines have been observed in individuals with pheochromocytoma, a neuroendocrine tumor. Measurement of metanephrines in urine is used to screen for the tumor. Analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS) is recommended due to the high sensitivity, specificity, and throughput of the technique. Herein, we describe an optimized LC-MS/MS assay for the analysis of urinary metanephrines.

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液相色谱串联质谱法分析尿中肾上腺素。

肾上腺素（甲肾上腺素[MN]和去甲肾上腺素[NMN]）分别是由儿茶酚胺、肾上腺素和去甲肾上腺素衍生的o -甲基化代谢物。在嗜铬细胞瘤（一种神经内分泌肿瘤）患者中观察到高浓度的肾上腺素。尿中肾上腺素的测定是用来筛选肿瘤的。建议使用液相色谱-串联质谱（LC-MS/MS）分析，因为该技术具有高灵敏度，特异性和通量。在此，我们描述了一种优化的LC-MS/MS分析尿肾上腺素的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.