Dissecting SNARE-Mediated Exocytosis in RBL-2H3 Mast Cells.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4314-3_10

Pratikshya Adhikari, Hao Xu

引用次数: 0

Abstract

SNARE-dependent mast cell (MC) exocytosis causes the release of a wide variety of mediators with important physiological/pathological consequences. Unlike synaptic transmission in the brain, which relies primarily on one set of exocytic SNAREs (i.e., Syntaxin1, SNAP-25, and VAMP2), MCs produce a multitude of exocytic SNAREs that can form a minimum of 8 distinct sets of fusogenic trans-SNARE complexes. Here we describe the genetic approaches we have developed to dissect the specific roles of these SNAREs in RBL-2H3 cells, a widely utilized model for studying MC signaling and exocytosis.

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剖析RBL-2H3肥大细胞中SNARE介导的外吞作用

snare依赖性肥大细胞（MC）胞吐引起多种介质的释放，具有重要的生理/病理后果。大脑中的突触传递主要依赖于一组胞外SNAREs（即Syntaxin1， SNAP-25和VAMP2），而MCs产生大量胞外SNAREs，可以形成至少8组不同的融合性trans-SNARE复合物。在这里，我们描述了我们开发的遗传学方法来剖析这些SNAREs在RBL-2H3细胞中的具体作用，RBL-2H3细胞是一个广泛使用的研究MC信号传导和胞吐的模型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.