Effects of periodontal disease on the proteomic profile of the periodontal ligament

IF 2.8 2区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of proteomics Pub Date : 2025-01-10 DOI:10.1016/j.jprot.2025.105384

Maria Eduarda Scordamaia Lopes , Camila Chierici Marcantonio , Cristiane Ribeiro Salmon , Luciana Souto Mofatto , Francisco Humberto Nociti Junior , Sigrun Eick , James Deschner , Joni Augusto Cirelli , Andressa Vilas Boas Nogueira

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Abstract

Periodontal disease affects over 1 billion people globally. This study investigated how periodontitis affects the protein profile of the periodontal ligament (PDL) in rats. Eight Holtzman rats were divided into control and experimental periodontitis groups. The PDL was isolated using laser capture microdissection and protein extracts were analyzed by mass spectrometry. Data analysis utilized specialized software, and Gene Ontology enrichment analysis identified significant protein functions. The data are available via ProteomeXchange with identifier PXD055817. Proteins such as SerpinB1, C5, and Lgals3 were validated through immunohistochemistry, and their gene expression was examined in an in vitro human PDL cell line. This study identified 1326 proteins, with 156 unique to the control group, 294 unique to the periodontitis group, and 876 common to both groups. Enrichment analysis revealed that proteins associated with the regulation of enzyme activity and RNA binding were significantly represented in the periodontitis group. There were increased levels of SerpinB1, C5, and Lgals3 in the periodontitis group based on proteomic and immunohistochemical analyses. Furthermore, these targets showed increased gene expression in stimulated human PDL cells. This study provides insights into the periodontitis-related alterations in the protein composition of the PDL and PDL cells, identifying both novel and previously known disease-associated proteins.

Significance

The periodontal ligament plays a crucial role in oral functions by providing structural support to the tooth. Due to the presence of undifferentiated mesenchymal cells, research into its regenerative capacity is ongoing. Pathological conditions can affect these functions and protein composition. Currently, there is a lack of comprehensive research specifically focusing on evaluating the periodontal ligament in both healthy and diseased states. This pioneering study screened for protein alterations and the mechanisms related to periodontitis. The possibility of using proteomic analysis to evaluate the protein alterations that occur in periodontitis, a disease with a high global incidence, could provide therapeutic targets and new biomarkers for future clinical studies.

Abstract Image

查看原文本刊更多论文

牙周病对牙周韧带蛋白质组学的影响。

全球有超过10亿人患有牙周病。本研究探讨了牙周炎对大鼠牙周韧带（PDL）蛋白谱的影响。将8只Holtzman大鼠分为对照组和实验性牙周炎组。用激光捕获显微解剖分离PDL，用质谱分析蛋白提取物。数据分析采用专门的软件，基因本体富集分析发现了重要的蛋白质功能。数据可通过ProteomeXchange获得，标识符为PXD055817。通过免疫组织化学验证了SerpinB1、C5和Lgals3等蛋白，并在体外人PDL细胞系中检测了它们的基因表达。这项研究确定了1326种蛋白质，其中156种是对照组特有的，294种是牙周炎组特有的，876种是两组共有的。富集分析显示，与酶活性调节和RNA结合相关的蛋白质在牙周炎组中显著存在。根据蛋白质组学和免疫组织化学分析，牙周炎组SerpinB1、C5和Lgals3水平升高。此外，这些靶点在受刺激的人PDL细胞中表现出增加的基因表达。这项研究提供了牙周炎相关的改变在PDL和PDL细胞的蛋白质组成的见解，确定了新的和以前已知的疾病相关蛋白。意义：牙周韧带为牙齿提供结构支撑，在口腔功能中起着至关重要的作用。由于存在未分化的间充质细胞，对其再生能力的研究正在进行中。病理条件会影响这些功能和蛋白质组成。目前，针对牙周韧带健康状态和病变状态的评估，还缺乏全面的研究。这项开创性的研究筛选了与牙周炎相关的蛋白质改变和机制。利用蛋白质组学分析来评估牙周炎（全球发病率高的疾病）中发生的蛋白质改变的可能性，可以为未来的临床研究提供治疗靶点和新的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of proteomics 生物-生化研究方法

CiteScore

7.10

自引率

3.00%

发文量

227

审稿时长

73 days

期刊介绍： Journal of Proteomics is aimed at protein scientists and analytical chemists in the field of proteomics, biomarker discovery, protein analytics, plant proteomics, microbial and animal proteomics, human studies, tissue imaging by mass spectrometry, non-conventional and non-model organism proteomics, and protein bioinformatics. The journal welcomes papers in new and upcoming areas such as metabolomics, genomics, systems biology, toxicogenomics, pharmacoproteomics. Journal of Proteomics unifies both fundamental scientists and clinicians, and includes translational research. Suggestions for reviews, webinars and thematic issues are welcome.