{"title":"V6 encoded by mulberry crinkle leaf virus is important for viral DNA replication.","authors":"Pei-Yu Han, Zhen-Ni Yin, Jing-Jing Yang, Meng-Si Zhang, Ying Huang, Jian Zhang, Quan-You Lu","doi":"10.1016/j.virol.2025.110392","DOIUrl":null,"url":null,"abstract":"<p><p>Mulberry crinkle leaf virus (MCLV) is a representative species of the genus Mulcrilevirus in the family Geminiviridae. Here, we identified an additional V6 ORF which embedded within the V4 ORF in the MCLV virion-sense strand. The expression of V6 was confirmed by analyzing the promoter activity of V6 ORF upstream sequences and quantifying the viral DNA accumulation in V6-mutant MCLV-infected tomato plants. Infectious clones of V6-mutant MCLV vII (pCA-1.1MCLV<sup>mv6</sup>) and V4 and V6 double-mutant MCLV vII (pCA-1.1MCLV<sup>mV4mV6</sup>) were constructed, and experiments transfecting protoplasts isolated from Nicotiana benthamiana and infecting tomato using the constructs were performed. The results indicate that V6 plays a critical role in the multiplication of MCLV in protoplasts, although it has minimal, if any, impact on the systemic infection of MCLV in plants. Additionally, V4 and V6 positively regulate the MCLV DNA replication in synergistic form.</p>","PeriodicalId":94266,"journal":{"name":"Virology","volume":"603 ","pages":"110392"},"PeriodicalIF":0.0000,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Virology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.virol.2025.110392","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Mulberry crinkle leaf virus (MCLV) is a representative species of the genus Mulcrilevirus in the family Geminiviridae. Here, we identified an additional V6 ORF which embedded within the V4 ORF in the MCLV virion-sense strand. The expression of V6 was confirmed by analyzing the promoter activity of V6 ORF upstream sequences and quantifying the viral DNA accumulation in V6-mutant MCLV-infected tomato plants. Infectious clones of V6-mutant MCLV vII (pCA-1.1MCLVmv6) and V4 and V6 double-mutant MCLV vII (pCA-1.1MCLVmV4mV6) were constructed, and experiments transfecting protoplasts isolated from Nicotiana benthamiana and infecting tomato using the constructs were performed. The results indicate that V6 plays a critical role in the multiplication of MCLV in protoplasts, although it has minimal, if any, impact on the systemic infection of MCLV in plants. Additionally, V4 and V6 positively regulate the MCLV DNA replication in synergistic form.
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