In vitro evaluation of anti-inflammatory, anti-oxidant activity of pomegranate peel extract mediated calcium sulfate nano particles.

Q3 Medicine

Medical Journal of Malaysia Pub Date : 2025-01-01

P Dathan, D Nallaswamy, S Rajeshkumar, S Joseph, I Shahin, M Tharani

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引用次数: 0

Abstract

Introduction: Pomegranate peel is considered a reservoir of biologically active compounds, the presence of which provides anti-inflammatory and antioxidant properties to peel extracts. Calcium sulfate is considered an ideal boneaugmenting material, and in the present study, pomegranate peel extract-mediated calcium sulfate nano particles (PPE CaSo4 NPs) were synthesized and their anti-inflammatory and antioxidant properties were evaluated. This study aimed to evaluate the biological effects of PPE CaSo4 NPs, with a focus on their anti-inflammatory and antioxidant properties. The objectives were to green - synthesize PPE CaSo4 NPs, analyze their optical characteristics using UV-visible spectra analysis, and subsequently evaluate their antioxidant and anti-inflammatory activities.

Materials and methods: UV-visible spectra analysis was employed to investigate the optical characteristics and surface morphology, such as the size and shape of PPE CaSo4 NPs synthesized at different time intervals, which were characterized using a Scanning Electron Microscope. Anti-inflammatory activity was evaluated using bovine serum albumin denaturation assay (BSA), and egg albumin denaturation assay (EA) was compared with diclofenac sodium as a standard. Antioxidant activity was measured using 2,2 Diphenyl -1- Picryl hydraxylhydrate assay (DPPH), hydrogen peroxide radical scavenging assay (H2O2), and Ferric Reducing Antioxidant power assay (FRAP). Comparison made with ascorbic acid as the standard.

Results: Anti-inflammatory activity was observed at all concentrations of PPE CaSo4 NPs, and there was no significant difference between the test material and the standard p>0.05. A significant difference was found for the antioxidant activity between PPE CaSo4 NPs and the standard in concentrations of 10 μl, for DPPH, 10 μl and 20 μl for H2O2 (p<0.05) between the concentrations of 30, 40, and 50, and there was no significant difference between the test material and the standard in all three tests conducted.

Conclusion: The study concluded that the PPE CaSo4 NPs have Anti-inflammatory and Antioxidant activities and are concentration-dependent.

本刊更多论文

石榴皮提取物介导的硫酸钙纳米颗粒抗炎、抗氧化活性的体外评价。

石榴皮被认为是生物活性化合物的储存库，其存在为石榴皮提取物提供抗炎和抗氧化特性。硫酸钙被认为是一种理想的增骨材料，本研究合成了石榴皮提取物介导的硫酸钙纳米颗粒（PPE CaSo4 NPs），并对其抗炎和抗氧化性能进行了评价。本研究旨在评估PPE CaSo4 NPs的生物学效应，重点研究其抗炎和抗氧化特性。目的是绿色合成PPE CaSo4 NPs，通过紫外可见光谱分析分析其光学特性，随后评估其抗氧化和抗炎活性。材料与方法：采用紫外可见光谱分析研究不同时间间隔合成的PPE CaSo4 NPs的光学特性和表面形貌，如尺寸、形状等，并利用扫描电镜对其进行表征。采用牛血清白蛋白变性试验（BSA）评估抗炎活性，并将鸡蛋白蛋白变性试验（EA）与双氯芬酸钠作为标准进行比较。采用2,2二苯基-1-苦基水合过氧化氢法（DPPH）、过氧化氢自由基清除法（H2O2）和铁还原抗氧化能力法（FRAP）测定抗氧化活性。以抗坏血酸为标准进行比较。结果：PPE CaSo4 NPs在各浓度下均具有抗炎活性，与标准材料p < 0.05无显著差异。在浓度为10 μl、DPPH浓度为10 μl、H2O2浓度为20 μl时，PPE CaSo4 NPs的抗氧化活性与标准有显著差异(p)。结论：PPE CaSo4 NPs具有抗炎和抗氧化活性，且具有浓度依赖性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Medical Journal of Malaysia Medicine-Medicine (all)

CiteScore

1.20

自引率

0.00%

发文量

165

期刊介绍： Published since 1890 this journal originated as the Journal of the Straits Medical Association. With the formation of the Malaysian Medical Association (MMA), the Journal became the official organ, supervised by an editorial board. Some of the early Hon. Editors were Mr. H.M. McGladdery (1960 - 1964), Dr. A.A. Sandosham (1965 - 1977), Prof. Paul C.Y. Chen (1977 - 1987). It is a scientific journal, published quarterly and can be found in medical libraries in many parts of the world. The Journal also enjoys the status of being listed in the Index Medicus, the internationally accepted reference index of medical journals. The editorial columns often reflect the Association''s views and attitudes towards medical problems in the country. The MJM aims to be a peer reviewed scientific journal of the highest quality. We want to ensure that whatever data is published is true and any opinion expressed important to medical science. We believe being Malaysian is our unique niche; our priority will be for scientific knowledge about diseases found in Malaysia and for the practice of medicine in Malaysia. The MJM will archive knowledge about the changing pattern of human diseases and our endeavours to overcome them. It will also document how medicine develops as a profession in the nation. We will communicate and co-operate with other scientific journals in Malaysia. We seek articles that are of educational value to doctors. We will consider all unsolicited articles submitted to the journal and will commission distinguished Malaysians to write relevant review articles. We want to help doctors make better decisions and be good at judging the value of scientific data. We want to help doctors write better, to be articulate and precise.