The effect of environmental factors on transepithelial potential in a model Amazonian teleost, the tambaqui (Colossoma macropomum): Implications for sodium balance in harsh environments.

Abstract

The tambaqui (Colossoma macropomum, G. Cuvier 1818) thrives both in the ion-poor waters of the Amazon and in commercial aquaculture. In both, environmental conditions can be harsh due to low ion levels, occasional high salt challenges (in aquaculture), low pH, extreme PO₂ levels (hypoxia and hyperoxia), high PCO₂ levels (hypercapnia), high ammonia levels (in aquaculture), and high and low temperatures. Ion transport across the gill is affected by active transport processes, passive diffusive permeability, ion concentrations (the chemical gradient), and transepithelial potential (TEP, the electrical gradient). The latter is a very important indicator of ionoregulatory status but is rarely measured. Using normoxic, normocapnic, ion-poor, low-dissolved organic carbon (DOC) well water (27°C, pH 7.0) as the acclimation and reference condition, we first confirmed that the strongly negative TEP (-22.3 mV inside relative to the external water) is a simple diffusion potential. We then evaluated the effects on TEP of more complex waters from the Rio Negro (strong hyperpolarization) and Rio Solimões (no significant change). Additionally, we have quantified significant effects of acute, realistic changes in environmental conditions-low pH (depolarization), hypercapnia (depolarization), hypoxia (depolarization), hyperoxia (hyperpolarization), elevated NaCl concentrations (depolarization), and elevated NH₄Cl concentrations (depolarization). The TEP responses help explain many of the changes in net Na⁺ flux rates reported in the literature. We have also shown marked effects of temperature on TEP and unidirectional Na⁺ flux rates (hyperpolarization and decreased fluxes at 21°C, depolarization and increased fluxes at 33°C) with no changes in net Na⁺ flux rates. Calculations based on the Nernst equation demonstrate the importance of the TEP changes in maintaining net Na⁺ balance.