The Relationship Between ADAMTS-4 and ADAMTS-5 Enzyme Levels in Patients With Degenerative Disc Disease: A Prospective Biochemical Study

IF 3.4 3区医学 Q1 ORTHOPEDICS

JOR Spine Pub Date : 2025-01-08 DOI:10.1002/jsp2.70037

Cansu Tuncer, Emin Mehmet Eminoğlu, Ömer Emre Yağlı, Gülçin Baş, Ahmet Sancar Topal, Ece Onur, Habib Özdemir, Şemsi Güliz Yılmaz, Gülgün Yılmaz Ovalı, Cüneyt Temiz

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Abstract

Study Design

Prospective biochemical study of comparison of A Disintegrin and Metalloproteinase with Thrombospondin motifs-4 (ADAMTS-4) and A Disintegrin and Metalloproteinase with Thrombospondin motifs 5 (ADAMTS5) levels in preoperative and postoperative venous blood, as well as in disc tissue obtained during surgery, in patients undergoing surgery for intervertebral disc disease, with enzyme levels in venous blood from a control group.

Objective

To compare the levels of ADAMTS-4 and ADAMTS-5 between patients with degenerative intervertebral discs and a healthy control group, aiming to identify biomarkers associated with intervertebral disc degeneration.

Literature

Although numerous studies have investigated the relationship between ADAMTS-4 and ADAMTS-5 enzymes and degeneration in experimental rat models and human tissues, no study has correlated their serum levels with intervertebral disc degeneration.

Method and Materials

Venous blood samples were obtained preoperatively and postoperatively from 41 patients (age: 42 ± 9.7 years, range 20–63) diagnosed with intervertebral disc disease. The affected disc levels were L4-L5 in 22 patients and L5-S1 in 19 patients. These patients were selected based on surgical indications due to radicular pain that persisted after an adequate course of conservative management, without any non-neurological deficit. Disc tissue samples were also obtained during surgery. Additionally, venous blood samples were collected from a control group with no diagnosed diseases, and lumbar MRIs of the control group showed no significant signs of degeneration. ADAMTS-4 and ADAMTS-5 levels were measured using the ELISA method on samples obtained after centrifugation of the collected blood and tissue specimens.

Results

The level of ADAMTS-4 in patient serum was found to be lower compared to the control group, while the level of ADAMTS-5 was higher in the patient serum and lower in the control group.

Conclusion

Elevated levels of ADAMTS-5 in the blood may be associated with intervertebral disc degeneration.

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退行性椎间盘病患者ADAMTS-4和ADAMTS-5酶水平的关系：一项前瞻性生化研究

研究设计：前瞻性生化研究，比较椎间盘疾病手术患者术前和术后静脉血以及手术中获得的椎间盘组织中A崩解素和金属蛋白酶与血栓反应蛋白基序-4 （ADAMTS-4）和A崩解素和金属蛋白酶与血栓反应蛋白基序- 5 （ADAMTS5）水平，并与对照组静脉血酶水平进行比较。目的：比较退行性椎间盘患者和健康对照组中ADAMTS-4和ADAMTS-5的水平，以确定与椎间盘退变相关的生物标志物。文献：虽然大量研究调查了ADAMTS-4和ADAMTS-5酶与实验大鼠模型和人体组织退行性变之间的关系，但没有研究将其血清水平与椎间盘退行性变联系起来。方法与材料：选取41例椎间盘病变患者（年龄42±9.7岁，年龄20 ~ 63岁）术前及术后静脉血标本。22例患者的椎间盘水平为L4-L5, 19例患者为L5-S1。这些患者是根据手术指征选择的，因为神经根疼痛在适当的保守治疗后仍然存在，没有任何非神经功能障碍。手术中也获得了椎间盘组织样本。此外，从没有确诊疾病的对照组收集静脉血样本，对照组的腰椎mri显示没有明显的变性迹象。对采集的血液和组织标本离心后的样品，采用ELISA法检测ADAMTS-4和ADAMTS-5的水平。结果：患者血清中ADAMTS-4水平低于对照组，而患者血清中ADAMTS-5水平高于对照组，低于对照组。结论：血液中ADAMTS-5水平升高可能与椎间盘退变有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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