Association of phospholipid transfer protein (PLTP) and the effect of genetic variant rs5072 on hypertriglyceridemia and atherogenic dyslipidemia in children and adolescents from Southeastern Mexico

IF 2.5 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinical biochemistry Pub Date : 2025-01-05 DOI:10.1016/j.clinbiochem.2024.110871

Luis E. Jiménez-Martínez , Anne Santerre , Héctor Ochoa-Díaz-López , Zendy Evelyn Olivo-Vidal , Itandehui Castro-Quezada , Cesar Antonio Irecta-Nájera

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引用次数: 0

Abstract

Introduction

Dyslipidemia is characterized by changes in lipid and lipoprotein levels in the blood where phospholipid transfer protein (PLTP) helps to regulate and modulate the size of high-density lipoproteins (HDL), working on the reverse transport of cholesterol. ApoA-1 is the primary protein component of HDL, and certain genetic variants like rs5072, have been associated with hypertriglyceridemia in children. This study aimed to explore the association between PLTP concentrations and the effect of the genetic variant APOA1 rs5072 on hypertriglyceridemia and atherogenic dyslipidemia (AD) in the pediatric population of Southeastern Mexico.

Materials and methods

A cross-sectional study was carried out with a case-control design for 364 pediatric patients between 2 and 17 years old in Chiapas and Tabasco, Mexico. Serum samples were used to evaluate PLTP concentrations using ELISA kits, and DNA from peripheral blood samples was used to study genetic variation using q-PCR with TaqMan® probes. For statistical analysis, Student t-test for media comparison, Chi-square for frequency and Pearson analysis for correlation was performed. The software SNPStats was used for inheritance models.

Results

Children with hypertriglyceridemia had higher levels of PLTP (8.3 ± 6.5 ng/ml) than the control group (6.4 ± 4.5 ng/ml). Similarly, the pediatric patients with AD had higher PLTP levels of 8.0 ± 6 ng/ml, mainly in children with high triglycerides who were between 10 and 17 years old (9.7 ± 8.0 ng/ml). Also, it was found that the genetic variant rs5072 had a protective effect against hypertriglyceridemia (OR = 0.61, p = 0.024) in the over-dominant inheritance model.

Conclusion

PLTP levels increase in pediatric patients aged 10 to 17 years with a diagnosis of hypertriglyceridemia and AD. The genetic variant rs5072 has a protective effect in hypertriglyceridemia.

查看原文本刊更多论文

墨西哥东南部儿童和青少年中磷脂转移蛋白（PLTP）和遗传变异rs5072对高甘油三酯血症和动脉粥样硬化性血脂异常的影响

简介：血脂异常的特征是血液中脂质和脂蛋白水平的变化，其中磷脂转移蛋白（PLTP）有助于调节和调节高密度脂蛋白（HDL）的大小，参与胆固醇的反向运输。ApoA-1是HDL的主要蛋白质成分，某些遗传变异如rs5072与儿童高甘油三酯血症有关。本研究旨在探讨PLTP浓度与遗传变异APOA1 rs5072对墨西哥东南部儿童高甘油三酯血症和动脉粥样硬化性血脂异常（AD）的影响之间的关系。材料和方法：采用病例对照设计对墨西哥恰帕斯州和塔巴斯科州364名2 - 17岁儿童进行横断面研究。采用ELISA试剂盒检测血清样本PLTP浓度，采用TaqMan®探针检测外周血样本DNA，采用q-PCR检测基因变异。在统计分析方面，媒介比较采用学生t检验，频率采用卡方检验，相关性采用Pearson分析。软件SNPStats用于继承模型。结果：高甘油三酯血症患儿PLTP水平（8.3±6.5 ng/ml）高于对照组（6.4±4.5 ng/ml）。同样，儿童AD患者PLTP水平较高，为8.0±6 ng/ml，主要发生在10 - 17岁的高甘油三酯儿童（9.7±8.0 ng/ml）。此外，在过显性遗传模型中，遗传变异rs5072对高甘油三酯血症具有保护作用（OR = 0.61, p = 0.024）。结论：在诊断为高甘油三酯血症和AD的10至17岁儿童患者中，PLTP水平升高。基因变异rs5072对高甘油三酯血症具有保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical biochemistry 医学-医学实验技术

CiteScore

5.10

自引率

0.00%

发文量

151

审稿时长

25 days

期刊介绍： Clinical Biochemistry publishes articles relating to clinical chemistry, molecular biology and genetics, therapeutic drug monitoring and toxicology, laboratory immunology and laboratory medicine in general, with the focus on analytical and clinical investigation of laboratory tests in humans used for diagnosis, prognosis, treatment and therapy, and monitoring of disease.