Induction of innate immunity and plant growth promotion in tomato unveils the antiviral nature of bacterial endophytes against groundnut bud necrosis virus.

IF 4 2区 医学 Q2 VIROLOGY
Journal of Virology Pub Date : 2025-02-25 Epub Date: 2024-12-31 DOI:10.1128/jvi.01803-24
R Sharanya, M Gayathri, P Renukadevi, N Saranya, M Suganthy, S Varanavasiappan, Amalendu Ghosh, S Nakkeeran
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引用次数: 0

Abstract

Tomato is an important crop worldwide, but groundnut bud necrosis virus (GBNV) often hampers its growth. This study investigates the antiviral potential of bacterial endophytes, including Brucella melitensis CNEB54, Bacillus licheniformis CNEB4, Bacillus velezensis CNEB26, and Bacillus vallismortis BAVE5 against GBNV, as well as their ability to enhance immunity and growth in tomato. All four bacterial isolates demonstrated a significant delay in GBNV symptom development 10 days post-inoculation, with disease incidence ranging from 18% to 36% compared to 84% in control. DAC-ELISA results indicated a noteworthy reduction in virus titer (0.32-0.96 OD) in treated tomato plants versus the control (3.26 OD). In addition, qPCR analysis revealed decreased viral copy numbers in plants treated with bacterial endophytes (1.3-3.1 × 105) as against in untreated inoculated control (2.4 × 106). Furthermore, these endophytes upregulated the expression of defense-associated genes, such as MAPKK1, PAL, PPO, LOX1, JAR1, and PDF 1.2. Field experiments with the application of B. melitensis and B. velezensis exhibited improved growth, with an average plant height of 123.70 cm, 14.87 flowers per plant, and a fruit weight of 549.3 g per plant, with a disease incidence of 18.1%. In comparison, the untreated control plants only reached a height of 104.73 cm, produced 11.17 flowers per plant, and yielded 267 g of fruit per plant, with a disease incidence of 30.1%. These findings strongly support the use of bacterial endophytes to reduce disease incidence and severity, enhance plant immunity and promote plant growth, resulting in overall crop productivity in sustainable agriculture.IMPORTANCEThe infection of GBNV in crops such as tomatoes, peanuts, and pulses leads to significant yield loss. Applying insecticides to control vector populations, can limit the spread of viruses carried by these vectors. The present study envisages a novel strategy to combat GBNV, with the help of bacterial endophytes. These bacterial endophytes have tremendously reduced the symptom expression of GBNV, induced the expression of defense genes during the tri-trophic interaction and promoted plant growth in tomatoes under field conditions. Hence, these bacteria are identified to be involved in immunity boosting, viral suppression and growth promotion.

诱导番茄的先天免疫和促进植物生长揭示了细菌内生菌对花生芽坏死病毒的抗病毒性质。
番茄是世界上重要的作物,但花生芽坏死病毒(GBNV)经常阻碍其生长。本研究探讨了番茄内生细菌Brucella melitensis CNEB54、Bacillus licheniformis CNEB4、Bacillus velezensis CNEB26和Bacillus vallismortis bae5对GBNV的抗病毒潜力,以及它们对番茄免疫和生长的促进作用。接种后10天,所有四种细菌分离株均表现出GBNV症状发展的显著延迟,疾病发病率从18%到36%不等,而对照组为84%。DAC-ELISA结果显示,与对照(3.26 OD)相比,处理番茄植株的病毒滴度显著降低(0.32 ~ 0.96 OD)。此外,qPCR分析显示,与未接种的对照(2.4 × 106)相比,接种细菌内生菌的植株(1.3 ~ 3.1 × 105)的病毒拷贝数减少。此外,这些内生菌上调了防御相关基因的表达,如MAPKK1、PAL、PPO、LOX1、JAR1和pdf1.2。田间试验结果表明,两种药剂均能促进植株生长,株高123.70 cm,单株花14.87朵,单株果重549.3 g,病害发生率为18.1%。对照株株高仅104.73 cm,单株开花11.17朵,单株果实267 g,发病率为30.1%。这些发现有力地支持了利用细菌内生菌来降低疾病发病率和严重程度、增强植物免疫力和促进植物生长,从而在可持续农业中提高作物的整体生产力。重要意义GBNV在番茄、花生和豆类等作物中的感染会导致显著的产量损失。使用杀虫剂控制病媒种群,可以限制这些病媒携带的病毒的传播。目前的研究设想了一种新的策略来对抗GBNV,在细菌内生菌的帮助下。这些细菌内生菌在田间条件下极大地降低了GBNV的症状表达,诱导了三营养相互作用中防御基因的表达,促进了番茄植株的生长。因此,这些细菌被确定参与免疫增强,病毒抑制和促进生长。
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来源期刊
Journal of Virology
Journal of Virology 医学-病毒学
CiteScore
10.10
自引率
7.40%
发文量
906
审稿时长
1 months
期刊介绍: Journal of Virology (JVI) explores the nature of the viruses of animals, archaea, bacteria, fungi, plants, and protozoa. We welcome papers on virion structure and assembly, viral genome replication and regulation of gene expression, genetic diversity and evolution, virus-cell interactions, cellular responses to infection, transformation and oncogenesis, gene delivery, viral pathogenesis and immunity, and vaccines and antiviral agents.
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