Proteomic study of the inhibitory effects of tannic acid on MRSA biofilm.

IF 4.4 2区 医学 Q1 PHARMACOLOGY & PHARMACY
Frontiers in Pharmacology Pub Date : 2024-12-18 eCollection Date: 2024-01-01 DOI:10.3389/fphar.2024.1413669
Yang Miao, Wang Shuang, Qu Qianwei, Liu Xin, Peng Wei, Yang Hai, Zhou Yonghui, Yu Xinbo
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引用次数: 0

Abstract

Introduction: The mechanism of tannic acid (TA) intervention on methicillin-resistant Staphylococcus aureus (MRSA, USA 300) biofilm formation was explored using proteomics.

Methods: The minimum inhibitory concentration (MIC) of TA against the MRSA standard strain USA 300 was determined by two-fold serial dilution of the microbroth. The effects of TA were studied using crystal violet staining. The morphology of TA-treated USA 300 cells was observed by scanning electron microscopy and confocal laser scanning microscopy. Differentially expressed proteins (DEPs) were screened using proteomic and biological information analyses, and their transcriptional levels were verified using real-time quantitative polymerase chain reaction.

Results: The MIC of TA was 0.625 mg/mL, whereas 1/2 MIC (0.3125 mg/mL) of TA significantly inhibited biofilm formation without affecting the bacterial growth (p < 0.01) and prevented the formation of a complete three-dimensional biofilm structure. Using 1/2 MIC of TA, 208 DEPs were identified, of which 127 were upregulated and 81 were downregulated. The transcriptional levels of the genes corresponding to five randomly selected DEPs (glnA, ribD, clpB, gap, and lukE) were consistent with the proteomics data (p < 0.05). Bioinformatic analysis showed that the changes in the MRSA strains after TA intervention primarily involved pyrimidine and purine metabolisms, arginine biosynthesis, and the citric acid cycle.

Conclusion: TA exerts an antibacterial effect on MRSA and can be used as a potential candidate for the development of anti-biofilm drugs, thereby laying a foundation for the treatment of MRSA biofilm-induced infections.

单宁酸对MRSA生物膜抑制作用的蛋白质组学研究。
前言:采用蛋白质组学方法探讨单宁酸(TA)干预耐甲氧西林金黄色葡萄球菌(MRSA, USA 300)生物膜形成的机制。方法:采用2倍连续稀释法测定TA对MRSA标准菌株us300的最低抑菌浓度。用结晶紫染色法研究TA的作用。用扫描电镜和激光共聚焦显微镜观察ta处理后USA 300细胞的形态。通过蛋白质组学和生物学信息分析筛选差异表达蛋白(DEPs),并通过实时定量聚合酶链反应验证其转录水平。结果:TA的MIC为0.625 mg/mL,而1/2 MIC (0.3125 mg/mL)的TA在不影响细菌生长的情况下显著抑制了生物膜的形成(p < 0.01),并阻止了完整三维生物膜结构的形成。利用TA的1/2 MIC,鉴定出208个dep,其中127个上调,81个下调。随机选择的5个DEPs (glnA、ribD、clpB、gap和lukE)对应基因的转录水平与蛋白质组学数据一致(p < 0.05)。生物信息学分析显示,TA干预后MRSA菌株的变化主要涉及嘧啶和嘌呤代谢、精氨酸生物合成和柠檬酸循环。结论:TA对MRSA具有抗菌作用,可作为开发抗生物膜药物的潜在候选物,为治疗MRSA生物膜诱导感染奠定基础。
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来源期刊
Frontiers in Pharmacology
Frontiers in Pharmacology PHARMACOLOGY & PHARMACY-
CiteScore
7.80
自引率
8.90%
发文量
5163
审稿时长
14 weeks
期刊介绍: Frontiers in Pharmacology is a leading journal in its field, publishing rigorously peer-reviewed research across disciplines, including basic and clinical pharmacology, medicinal chemistry, pharmacy and toxicology. Field Chief Editor Heike Wulff at UC Davis is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
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