Identification and Characterisation of Potential Targets for N6-methyladenosine (m6A) Modification during Intervertebral Disc Degeneration.

IF 3.3 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Frontiers in bioscience (Landmark edition) Pub Date : 2024-11-28 DOI:10.31083/j.fbl2912405

Jianlin Shen, Qiang Zhang, Yujian Lan, Qingping Peng, Ziyu Ji, Yanjiao Wu, Huan Liu

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Abstract

Background: The mechanism for RNA methylation during disc degeneration is unclear. The aim of this study was to identify N6-methyladenosine (m6A) markers and therapeutic targets for the prevention and treatment of intervertebral disc degeneration (IDD).

Methods: Methylated RNA immunoprecipitation sequencing (MeRIP-seq) and quantitative reverse transcription PCR (RT-qPCR) were employed to analyze m6A modifications of IDD-related gene expression. Bioinformatics was used to identify enriched gene pathways in IDD. m6A-RIP-qPCR was used to validate potential targets and markers.

Results and conclusion: Human IDD samples exhibited a distinct m6A modification pattern that allowed associated genes and pathways to be identified. These genes had functions such as "nuclear factor kappa-B (NF-κB) binding" and "extracellular matrix components", which are crucial for IDD pathogenesis. ANXA2 showed increased m6A modification in IDD, while SLC3A2 and PBX3 showed decreased m6A methylation. The results of this study offer novel insights for the prevention and treatment of IDD.

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椎间盘退变过程中n6 -甲基腺苷（m6A）修饰的潜在靶点的鉴定和表征。

背景：椎间盘退变过程中RNA甲基化的机制尚不清楚。本研究旨在确定N6-甲基腺苷（m6A）标记物和治疗靶点，以预防和治疗椎间盘退变（IDD）：方法：采用甲基化RNA免疫沉淀测序（MeRIP-seq）和定量反转录PCR（RT-qPCR）分析IDD相关基因表达的m6A修饰。m6A-RIP-qPCR 被用来验证潜在的靶点和标记物：人类 IDD 样本表现出独特的 m6A 修饰模式，从而确定了相关基因和通路。这些基因具有 "核因子卡巴-B（NF-κB）结合 "和 "细胞外基质成分 "等功能，对IDD发病机制至关重要。ANXA2在IDD中的m6A修饰增加，而SLC3A2和PBX3的m6A甲基化减少。这项研究的结果为预防和治疗 IDD 提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in bioscience (Landmark edition)

CiteScore

3.50

自引率

0.00%

发文量