Expanding horizons: genetic code expansion technology in the study of PTM functions.

IF 3.3 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioorganic & Medicinal Chemistry Pub Date : 2025-02-01 Epub Date: 2024-12-19 DOI:10.1016/j.bmc.2024.118049

Jingzhuo Chen, Hui Ye

引用次数: 0

Abstract

Recent advancements in Genetic Code Expansion (GCE) have significantly enhanced our understanding of post-translational modifications (PTMs), which are critical for protein regulation. GCE facilitates the precise incorporation of unnatural amino acids (UAAs) at specific sites within proteins of interest (POIs), making it a powerful tool for modulating PTMs in vivo. This review summarizes the various UAAs utilized to directly incorporate PTMs into proteins through GCE, with a focus on their applications in both histone and non-histone PTMs research. We also discuss the challenges associated with incorporating certain PTMs into target proteins via GCE and provide an overview of the latest strategies developed to overcome these hurdles.

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拓展视野：PTM功能研究中的遗传密码扩展技术。

遗传密码扩展（GCE）的最新进展极大地增强了我们对翻译后修饰（PTMs）的理解，这对蛋白质调控至关重要。GCE促进了非天然氨基酸（UAAs）在目标蛋白（POIs）特定位点的精确结合，使其成为体内调节PTMs的有力工具。本文综述了通过GCE将PTMs直接结合到蛋白质中的各种uaa，重点介绍了它们在组蛋白和非组蛋白PTMs研究中的应用。我们还讨论了通过GCE将某些ptm纳入靶蛋白的挑战，并概述了为克服这些障碍而开发的最新策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioorganic & Medicinal Chemistry 医学-生化与分子生物学

CiteScore

6.80

自引率

2.90%

发文量

413

审稿时长

17 days

期刊介绍： Bioorganic & Medicinal Chemistry provides an international forum for the publication of full original research papers and critical reviews on molecular interactions in key biological targets such as receptors, channels, enzymes, nucleotides, lipids and saccharides. The aim of the journal is to promote a better understanding at the molecular level of life processes, and living organisms, as well as the interaction of these with chemical agents. A special feature will be that colour illustrations will be reproduced at no charge to the author, provided that the Editor agrees that colour is essential to the information content of the illustration in question.