Determination of optimum probiotic dosage for the culture of whiteleg shrimp, Litopenaeus vannamei in an indoor system

IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX Pub Date : 2024-12-01 DOI:10.1016/j.mex.2024.103076

Edward Terhemen Akange , Benjamin Orfega Kwaghvihi , Olumide A. Odeyemi , Hajar Rastegari , Muhamad Shukri Shafie , Ahmad Ideris Abdul Rahim , Amyra Suryatie Kamaruzzan , Siti Rozaimah Sheikh Abdullah , Nor Azman Kasan

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Abstract

Determining the optimum application dosage of probiotic in biofloc system is often challenging because the microbial community seems to exert similar effects irrespective of their dosages. It is however noted that certain dosages promote higher yield in shrimp culture more effectively. Principal component analysis was adopted to identify these optimum dosages where 1-way ANOVA could not clearly identify due to the effects of microbial community. The effect of varying application dosages of probiotics in a shrimp culture system on growth indices and water quality variables were studied in a culture trial of Litopenaeus vannamei that lasted for 84 days in an indoor biofloc culture system. Only 4 (26.66 %) of variables showed significant difference in the 1-way ANOVA conducted. Principal Component Analysis (PCA) was used to illustrate the dimensional interactions among variables. Contributions of observation points at 7 ml.l^-1 (Obs1–3), 14 ml.l^-1 (Obs 4–6), and 21 ml.l^-1 (Obs 7–9) were analyzed. The Obs 4–6 (representing 14 ml.l^-1) had the highest mean contributory effect (11.03) indicating the greatest impact recorded in the PCA relationships among growth indices and water quality variables. It was concluded that probiotic dosages can be determined in biofloc system based on the contributory effect it enhances in the measured variables.

•
Microbial community in biofloc affects the clear distinctions of the dosages due to proliferations
•
1-way ANOVA alone may not show dosage-related variations.
•
PCA could identify the optimum probiotic dosage that would enhance shrimp growth performance and maintain favourable water quality conditions in biofloc aquaculture systems.

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凡纳滨对虾室内培养最佳益生菌用量的确定。

确定益生菌在生物絮团系统中的最佳应用剂量通常是具有挑战性的，因为微生物群落似乎无论其剂量如何都具有相似的效果。然而，注意到某些剂量更有效地促进对虾养殖的高产。由于微生物群落的影响，单因素方差分析无法明确确定最佳剂量，采用主成分分析确定最佳剂量。在室内生物群落培养系统中进行了84 d的凡纳滨对虾（Litopenaeus vannamei）培养试验，研究了不同剂量益生菌对对虾生长指标和水质指标的影响。在进行的单因素方差分析中，只有4个（26.66%）变量显示有显著差异。采用主成分分析（PCA）来说明变量之间的维度交互作用。分析了7 ml.l-1 （Obs1-3）、14 ml.l-1 （Obs 4-6）和21 ml.l-1 （Obs 7-9）观测点的贡献。Obs 4-6（代表14 ml.l-1）的平均贡献效应最高（11.03），表明在生长指数和水质变量的主成分分析关系中影响最大。综上所述，可以根据益生菌对被测变量的贡献效应来确定菌群系统中益生菌的剂量。•生物絮团中的微生物群落由于增殖而影响剂量的明确区分•单因素方差分析可能无法显示剂量相关的变化。•主成分分析法可以确定生物絮团养殖系统中提高对虾生长性能和维持良好水质条件的最佳益生菌剂量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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