Sir2 is required for the quiescence-specific condensed three-dimensional chromatin structure of rDNA.

Christine Cucinotta, Rachel Dell, Kris Alavattam, Toshio Tsukiyama
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Abstract

Quiescence in Saccharomyces cerevisiae is a reversible G0 crucial for long-term survival under nutrient-deprived conditions. During quiescence, the genome is hypoacetylated and chromatin undergoes significant compaction. However, the 3D structure of the ribosomal DNA (rDNA) locus in this state is not well understood. Here, we report that the rDNA locus in quiescent cells forms a distinct condensed loop-like structure, different from structures observed during the mitotic cell cycle. Deletion of SIR2 disrupts this structure, causing it to collapse into a small dot and resulting in quiescence entry and exit defects. In contrast, Sir2 affects rDNA structure only modestly in G2/M phase. In the absence of Sir2, occupancy of both RNA Polymerase II and histone H3 increase at the rDNA locus during quiescence and through quiescence exit, further indicating gross defects in chromatin structure. Together, these results uncover a previously undescribed rDNA chromatin structure specific to quiescent cells and underscore the importance of Sir2 in facilitating the transition between cellular states.

Sir2对于rDNA的静止特异性浓缩三维染色质结构是必需的。
酿酒酵母的静止是一种可逆的g0,对于营养剥夺条件下的长期生存至关重要。在静止期间,基因组被低乙酰化,染色质被显著压缩。然而,在这种状态下核糖体DNA (rDNA)位点的三维结构尚不清楚。在这里,我们报告了静止细胞中的rDNA位点形成一个独特的浓缩环状结构,不同于有丝分裂细胞周期中观察到的结构。SIR2的缺失破坏了这种结构,使其坍缩成一个小点,并导致静止进入和退出缺陷。相反,Sir2仅在G2/M期轻微影响rDNA结构。在Sir2缺失的情况下,RNA聚合酶II和组蛋白H3在rDNA位点的占用率在休眠期间和休眠退出时都有所增加,进一步表明染色质结构存在严重缺陷。总之,这些结果揭示了以前未描述的静止细胞特异性rDNA染色质结构,并强调了Sir2在促进细胞状态之间转换中的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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