The expression of Siglec-10 on naive B cells is involved in the pathology of systemic lupus erythematosus.

IF 3.4 4区医学 Q2 RHEUMATOLOGY

Clinical and experimental rheumatology Pub Date : 2024-12-21 DOI:10.55563/clinexprheumatol/hlnpiz

Bomiao Ju, Jing Luo, Nan Hu, Jing Zhang, Li Zhu, Qian Li, Yanhua Wang, Jing Huang, Qi An, Qianyun Xu, Zhiming Hao, Dan Pu, Xiaohong Lv, Xin Li, Yongwei Huo, Baojun Zhang, Lan He

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引用次数: 0

Abstract

Objectives: Previous studies have reported the expansion of CD19+Siglec-10+ B cells in systemic lupus erythematosus (SLE) patients. However, the composition of this cell population, phenotype and characteristics are still unknown.

Methods: We examined this memory B-cell subset's composition and phenotype and determined the SYK and AKT phosphorylation levels by flow cytometry. Additionally, we explored the relationship between Siglec-10 expression on B-cell subsets and clinical manifestations.

Results: Our results indicated elevated levels of Siglec-10 on naive B cells in active SLE patients. Compared with healthy controls (HCs) and inactive SLE patients, the Siglec-10+ B cells in active SLE patients exhibited elevated CD40 and CD21low levels. The levels of Siglec-10 on naive B cells were positively correlated with the proportion of CD21low double negative (DN) B cells and the SLEDAI-2K score.

Conclusions: The results indicate that the upregulation of Siglec-10+/naive B cells may function as a feedback mechanism to regulate B cell hyperreactivity. Monitoring the proportion of Siglec-10+/naive B cells may contribute to the evaluation of disease progression in SLE.

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siglece -10在幼稚B细胞上的表达参与系统性红斑狼疮的病理过程。

目的：先前的研究报道了系统性红斑狼疮（SLE）患者中CD19+ siglece -10+ B细胞的扩增。然而，该细胞群的组成、表型和特征仍然未知。方法：我们检测了记忆b细胞亚群的组成和表型，并通过流式细胞术检测了SYK和AKT的磷酸化水平。此外，我们还探讨了b细胞亚群中siglece -10的表达与临床表现之间的关系。结果：我们的研究结果表明活动性SLE患者初始B细胞siglece -10水平升高。与健康对照（hc）和非活动性SLE患者相比，活动性SLE患者的siglece -10+ B细胞CD40和CD21low水平升高。幼稚B细胞上siglece -10水平与CD21low双阴性（DN） B细胞比例和SLEDAI-2K评分呈正相关。结论：结果提示siglece -10+/naive B细胞上调可能作为一种反馈机制调节B细胞的高反应性。监测siglece -10+/幼稚B细胞的比例可能有助于评估SLE的疾病进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical and experimental rheumatology 医学-风湿病学

CiteScore

6.10

自引率

18.90%

发文量

377

审稿时长

3-6 weeks

期刊介绍： Clinical and Experimental Rheumatology is a bi-monthly international peer-reviewed journal which has been covering all clinical, experimental and translational aspects of musculoskeletal, arthritic and connective tissue diseases since 1983.