Mild-to-moderate psoriasis is associated with subclinical inflammation in the duodenum and a tendency of disturbed intestinal barrier

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimica et biophysica acta. Molecular basis of disease Pub Date : 2024-12-19 DOI:10.1016/j.bbadis.2024.167634

Patrik Lundquist , Eva Hagforsen , Michael Wagner , Mohammad Alimohammadi , Fabio Rabelo Melo , Gunnar Pejler , Per Artursson , Marie Carlson , Ola Rollman , Maria Lampinen

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Abstract

Psoriasis is a chronic skin disease occasionally associated with abdominal symptoms and IBD. We aimed to characterize intestinal immune cells and the integrity of the intestinal barrier in psoriasis. Biopsies from the duodenum and colon were analyzed by flow cytometry and immunohistochemistry for the presence and activation status of different immune cell populations. Intestinal permeability was measured using Ussing chambers. Proinflammatory markers were analyzed in fecal and blood samples using ELISA. The intestinal level of inflammatory mediators was assessed using a multiplex proximity extension assay. We found an increased density of intestinal eosinophils, mast cells, macrophages, and CD8⁺ T-cells in psoriasis; eosinophils, macrophages, and CD8+ T-cells expressed activation markers. Half of the psoriasis patients showed increased permeability across the duodenum, correlating with increased mucosal IL-17A, IL-13, IL-2, and IL-20, and with gastrointestinal symptoms. Our findings reveal that psoriasis is associated with low-grade intestinal inflammation, which may contribute to abdominal symptoms in these patients and possibly set the stage for the development of intestinal disease.

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轻至中度牛皮癣与十二指肠亚临床炎症和肠屏障紊乱的倾向有关。

牛皮癣是一种慢性皮肤病，偶尔伴有腹部症状和IBD。我们的目的是表征牛皮癣的肠道免疫细胞和肠道屏障的完整性。通过流式细胞术和免疫组织化学分析了十二指肠和结肠活检中不同免疫细胞群的存在和激活状态。采用ususing chamber测定肠通透性。采用酶联免疫吸附法分析粪便和血液中的促炎标志物。肠道炎症介质水平的评估使用多重接近扩展试验。我们发现牛皮癣患者肠道嗜酸性粒细胞、肥大细胞、巨噬细胞和CD8+ t细胞密度增加；嗜酸性粒细胞、巨噬细胞和CD8+ t细胞表达活化标记物。一半的银屑病患者表现为十二指肠通透性增加，与粘膜IL-17A、IL-13、IL-2和IL-20增加以及胃肠道症状相关。我们的研究结果表明，牛皮癣与低级别肠道炎症有关，这可能导致这些患者出现腹部症状，并可能为肠道疾病的发展奠定基础。

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来源期刊

Biochimica et biophysica acta. Molecular basis of disease 生物-生化与分子生物学

CiteScore

12.30

自引率

0.00%

发文量

218

审稿时长

32 days

期刊介绍： BBA Molecular Basis of Disease addresses the biochemistry and molecular genetics of disease processes and models of human disease. This journal covers aspects of aging, cancer, metabolic-, neurological-, and immunological-based disease. Manuscripts focused on using animal models to elucidate biochemical and mechanistic insight in each of these conditions, are particularly encouraged. Manuscripts should emphasize the underlying mechanisms of disease pathways and provide novel contributions to the understanding and/or treatment of these disorders. Highly descriptive and method development submissions may be declined without full review. The submission of uninvited reviews to BBA - Molecular Basis of Disease is strongly discouraged, and any such uninvited review should be accompanied by a coverletter outlining the compelling reasons why the review should be considered.