Unveiling Diversity and Function: Venom-Associated Microbes in Two Spiders, Heteropoda venatoria and Chilobrachys guangxiensis.

IF 3.3 3区生物学 Q2 ECOLOGY

Microbial Ecology Pub Date : 2024-12-21 DOI:10.1007/s00248-024-02476-y

Likun Zhao, Shanfeng Zhang, Jingchen Li, Chao Zhang, Ruoyi Xiao, Xinyuan Bai, Hongkang Xu, Feng Zhang

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引用次数: 0

Abstract

Spiders are natural predators of agricultural pests, primarily due to the potent venom in their venom glands. Spider venom is compositionally complex and holds research value. This study analyzes the diversity of symbiotic bacteria in spider venom glands and venom, as well as the biological activity of culturable symbiotic bacteria. Focusing on the venom glands and venom of Heteropoda venatoria and Chilobrachys guangxiensis, we identified a diverse array of microorganisms. High-throughput sequencing detected 2151 amplicon sequence variants (ASVs), spanning 31 phyla, 75 classes, and 617 genera. A total of 125 strains of cultivable bacteria were isolated. Using the Oxford cup method, crude extracts from 46 of these strains exhibited inhibitory effects against at least one indicator bacterium. MTT (Thiazolyl blue) assays revealed that the crude extracts from 43 strains had inhibitory effects on tumor cell line MGC-803 growth. Additionally, DAPI (4',6-diamidino-2'-phenylindole) staining and flow cytometry were employed to detect cell apoptosis. The anti-inflammatory activity of nine bacterial strains was assessed using a NO assay kit and enzyme-linked immunosorbent assay (ELISA). This study further investigated the biological activity of venom, exploring the relationship between the venom and the functional activity of venom-associated bacteria.

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揭示多样性和功能：两种蜘蛛——venatorheteropoda and Chilobrachys guangxiensis的毒液相关微生物。

蜘蛛是农业害虫的天然捕食者，主要是因为它们的毒腺中有强大的毒液。蜘蛛毒液成分复杂，具有研究价值。本研究分析了蜘蛛毒腺和毒液中共生细菌的多样性，以及可培养共生细菌的生物活性。我们以蛇毒腺和毒液为研究对象，鉴定出多种微生物。高通量测序检测到2151个扩增子序列变异（asv），跨越31门、75纲、617属。共分离出125株可培养细菌。使用牛津杯法，这些菌株的46个粗提取物对至少一种指示细菌表现出抑制作用。MTT （Thiazolyl blue）检测结果表明，43株菌株粗提物对肿瘤细胞株MGC-803的生长均有抑制作用。采用DAPI（4′，6-二氨基-2′-苯基吲哚）染色和流式细胞术检测细胞凋亡。采用NO测定试剂盒和酶联免疫吸附试验（ELISA）评价9株细菌的抗炎活性。本研究进一步研究了蛇毒的生物活性，探讨了蛇毒与蛇毒相关细菌功能活性之间的关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbial Ecology 生物-海洋与淡水生物学

CiteScore

6.90

自引率

2.80%

发文量

212

审稿时长

3-8 weeks

期刊介绍： The journal Microbial Ecology was founded more than 50 years ago by Dr. Ralph Mitchell, Gordon McKay Professor of Applied Biology at Harvard University in Cambridge, MA. The journal has evolved to become a premier location for the presentation of manuscripts that represent advances in the field of microbial ecology. The journal has become a dedicated international forum for the presentation of high-quality scientific investigations of how microorganisms interact with their environment, with each other and with their hosts. Microbial Ecology offers articles of original research in full paper and note formats, as well as brief reviews and topical position papers.