{"title":"A novel dicyanoisophorone-based fluorescent probe for rapid detection of acetylcholinesterase in biological systems.","authors":"Yuran Gui, Jingran Wang, Quan Gou, Xin Yu, Yan Yang, Chen Wang, Liping Li, Wanxia Gao, Wei Liu, Hua Wang, Xiji Shu, Yibin Zhang, Jinting Shang","doi":"10.1016/j.saa.2024.125587","DOIUrl":null,"url":null,"abstract":"<p><p>Acetylcholinesterase (AChE) plays a vital role in various neurological diseases including brain disorders, neurotransmission alterations, and cancer. Developing effective methods to image AChE in biological samples is essential for understanding its mechanisms in biosystems. Here, we introduce a novel fluorescent probe CNA, that enables detection of AChE at 520 nm with rapid response time of 60 s and a detection limit of 0.014 U/mL. We successfully applied CNA to image endogenous and exogenous AChE in PC12 cells and in living mice. These findings highlight the potential of CNA as an effective method to study the physiological and pathological roles of AChE in complex living systems.</p>","PeriodicalId":94213,"journal":{"name":"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy","volume":"329 ","pages":"125587"},"PeriodicalIF":0.0000,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.saa.2024.125587","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Acetylcholinesterase (AChE) plays a vital role in various neurological diseases including brain disorders, neurotransmission alterations, and cancer. Developing effective methods to image AChE in biological samples is essential for understanding its mechanisms in biosystems. Here, we introduce a novel fluorescent probe CNA, that enables detection of AChE at 520 nm with rapid response time of 60 s and a detection limit of 0.014 U/mL. We successfully applied CNA to image endogenous and exogenous AChE in PC12 cells and in living mice. These findings highlight the potential of CNA as an effective method to study the physiological and pathological roles of AChE in complex living systems.
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