Genome-wide identification and expression analysis of the kinesin gene superfamily suggests roles in response to abiotic stress and fertility of wheat (Triticum aestivum L.).

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Qinge Chen, Yang Ren, Qin Yan, Zhiyuan Zheng, Gaisheng Zhang, Lingjian Ma, Qilu Song, Na Niu
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Abstract

Background: Kinesin is a motor for microtubule-based motility. It plays a vital role in plant growth and development. The kinesin superfamily members are known mainly from Arabidopsis. Little research about kinesin superfamily has been conducted on hexploid wheat (Triticum aestivum L.). The functions of kinesins in wheat growth and development, regulation of cell division and response to stress are still unclear.

Results: In this study, we identified 155 kinesin (TaKIN) genes in wheat, which were divided into 10 families and some orphan genes via phylogenetic analysis. Less gene structural differences showed that TaKIN genes had redundant functions. The conserved domains of different family members were different, and some families might have some special functional domains. We found many cis-acting elements related to hormones (GA, Auxin, SA, MeJA), cell cycle and cell division in homeopathic elements of the TaKIN genes. Collinearity analysis showed that TaKIN genes were more conservative in monocotyledons. Expression level in different tissues at different stages suggested that TaKIN family may function during the whole growth and development process in wheat. It was worth noting there were quite different at gene expression level between physiological and heritable male sterile lines during the different stages of pollen development. The differential expression patterns of some TaKIN genes between male sterile line and maintainer line might be related to wheat male sterility. Furthermore, we also found TaKIN genes were involved in response to plant hormones and abiotic stress by stress assays.

Conclusions: The result is useful for further exploration of the molecular mechanism of kinesin genes in wheat male sterility and provides important information concerning response to plant hormones and abiotic stress caused by kinesin genes.

驱动蛋白基因超家族的全基因组鉴定和表达分析表明了其在小麦(Triticum aestivum L. )非生物胁迫响应和生育中的作用。
背景:Kinesin是一种基于微管的运动马达。它在植物生长发育中起着至关重要的作用。激酶超家族成员主要来自拟南芥。关于半胚小麦(Triticum aestivum L.)的酪蛋白超家族的研究很少。驱动蛋白在小麦生长发育、细胞分裂调控和逆境应答中的作用尚不清楚。结果:本研究通过系统发育分析,鉴定出155个小麦驱动蛋白(TaKIN)基因,这些基因被划分为10个家族和一些孤儿基因。基因结构差异较小表明TaKIN基因具有冗余功能。不同家族成员的保守结构域是不同的,有些家族可能有一些特殊的功能结构域。我们在TaKIN基因的顺势疗法元件中发现了许多与激素(GA、生长素、SA、MeJA)、细胞周期和细胞分裂相关的顺式作用元件。共线性分析表明,TaKIN基因在单子叶植物中较为保守。不同组织、不同阶段的表达水平表明,TaKIN家族可能在小麦的整个生长发育过程中发挥作用。值得注意的是,在花粉发育的不同阶段,生理不育系与遗传不育系之间的基因表达水平存在较大差异。一些TaKIN基因在雄性不育系和保持系之间的差异表达模式可能与小麦雄性不育有关。此外,我们还通过胁迫实验发现TaKIN基因参与了植物对激素和非生物胁迫的响应。结论:该结果为进一步探索小麦雄性不育中激酶基因的分子机制提供了依据,并为小麦对植物激素和激酶基因引起的非生物胁迫的响应提供了重要信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Genomics
BMC Genomics 生物-生物工程与应用微生物
CiteScore
7.40
自引率
4.50%
发文量
769
审稿时长
6.4 months
期刊介绍: BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.
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