Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations.

IF 2.5 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Progress Pub Date : 2024-12-20 DOI:10.1002/btpr.3526

Laura Keitel, Benjamin Schick, Gino Pohen, Stanislav Yordanov, Jochen Büchs

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引用次数: 0

Abstract

Phocaeicola vulgatus (formerly Bacteroides vulgatus), an anaerobic gut bacterium, produces several organic acids. Research on P. vulgatus is still in its infancy. However, a detailed understanding of P. vulgatus growth and metabolism is essential for its assessment as an organic acid producer. Media variations, including different initial glucose and NH₄Cl concentrations and osmolalities, are significant means to yield higher organic acid titers. Furthermore, examining different nitrogen and carbon sources is important to evaluate the potential of P. vulgatus for growth on renewable resources. Cultivations were performed in an in-house built device for anaerobic online-monitoring of fluorescence and scattered light in microtiter plates. Results revealed that the highest organic acid concentrations were reached while using galactose, glucose, or xylose as a carbon source, high osmolalities, and 0.25 g L^-1 NH₄Cl. In addition, the organic acid composition changed with changing carbon and nitrogen sources. P. vulgatus was successfully further characterized, thereby contributing to a faster characterization of other anaerobic strains and paving the way for anaerobic organic acid production.

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利用厌氧微孔板培养在线监测 Phocaeicola vulgatus 生产有机酸的特征。

普通Phocaeicola vulgatus（原普通拟杆菌），一种厌氧肠道细菌，产生几种有机酸。目前，对其的研究还处于初级阶段。然而，详细了解其生长和代谢是评估其作为有机酸生产者的必要条件。培养基的变化，包括不同的初始葡萄糖和NH4Cl浓度和渗透压，是产生更高有机酸滴度的重要手段。此外，研究不同的氮碳源对评价普通胡杨在可再生资源上的生长潜力具有重要意义。培养是在一个内部建立的装置进行厌氧在线监测荧光和散射光在微量滴度板。结果表明，当使用半乳糖、葡萄糖或木糖作为碳源，高渗透压和0.25 g L-1 NH4Cl时，有机酸浓度达到最高。此外，有机酸组成随碳氮源的变化而变化。P. vulgatus的进一步表征成功，从而有助于更快地表征其他厌氧菌株，并为厌氧有机酸的生产铺平道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Progress 工程技术-生物工程与应用微生物

CiteScore

6.50

自引率

3.40%

发文量

审稿时长

4 months

期刊介绍： Biotechnology Progress , an official, bimonthly publication of the American Institute of Chemical Engineers and its technological community, the Society for Biological Engineering, features peer-reviewed research articles, reviews, and descriptions of emerging techniques for the development and design of new processes, products, and devices for the biotechnology, biopharmaceutical and bioprocess industries. Widespread interest includes application of biological and engineering principles in fields such as applied cellular physiology and metabolic engineering, biocatalysis and bioreactor design, bioseparations and downstream processing, cell culture and tissue engineering, biosensors and process control, bioinformatics and systems biology, biomaterials and artificial organs, stem cell biology and genetics, and plant biology and food science. Manuscripts concerning the design of related processes, products, or devices are also encouraged. Four types of manuscripts are printed in the Journal: Research Papers, Topical or Review Papers, Letters to the Editor, and R & D Notes.