3D Fluorescence Spectroscopy Combined With Chemometrics as a Tool for Control of Imprinted Protein Purification From Template Molecules

IF 2.3 4区 化学 Q1 SOCIAL WORK
Natalia A. Burmistrova, Polina M. Ilicheva, Kirill Yu. Presnyakov, Pavel S. Pidenko, Douglas N. Rutledge
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引用次数: 0

Abstract

Imprinted proteins (IPs) are promising alternatives to natural recognition systems, such as biological receptors or antibodies. One of the crucial stages during development of IPs is removal of the template molecules from its complex with the protein. In this study, bovine serum albumin was imprinted in the presence of 4-hydroxycoumarin (4-HC); purification of IPs were carried out by dialysis, and fluorescence 3D spectroscopy was used to monitor the IP purification process. Excitation–emission matrix (EEM) was further investigated via several chemometric algorithms (principal component analysis [PCA], parallel factor analysis [PARAFAC], and independent components analysis [ICA]). We found that the models using PARAFAC and ICA worked better than those of PCA. It was shown that PARAFAC and ICA analyses allow not only to recognize IP sample with signal close to nonimprinted protein, but also to provide recommendations on the optimal dialysis time.

三维荧光光谱结合化学计量学作为模板分子印迹蛋白纯化控制工具
印迹蛋白(IPs)是自然识别系统(如生物受体或抗体)的有希望的替代品。IPs发育的关键阶段之一是将模板分子从其与蛋白质的复合物中去除。在这项研究中,牛血清白蛋白在4-羟基香豆素(4-HC)的存在下被印迹;通过透析纯化IPs,并利用荧光三维光谱监测IP纯化过程。通过几种化学计量学算法(主成分分析[PCA]、平行因子分析[PARAFAC]和独立成分分析[ICA])进一步研究了激发-发射矩阵(EEM)。我们发现使用PARAFAC和ICA的模型比PCA的模型效果更好。结果表明,PARAFAC和ICA分析不仅可以识别信号接近非印迹蛋白的IP样品,而且还可以提供最佳透析时间的建议。
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来源期刊
Journal of Chemometrics
Journal of Chemometrics 化学-分析化学
CiteScore
5.20
自引率
8.30%
发文量
78
审稿时长
2 months
期刊介绍: The Journal of Chemometrics is devoted to the rapid publication of original scientific papers, reviews and short communications on fundamental and applied aspects of chemometrics. It also provides a forum for the exchange of information on meetings and other news relevant to the growing community of scientists who are interested in chemometrics and its applications. Short, critical review papers are a particularly important feature of the journal, in view of the multidisciplinary readership at which it is aimed.
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