Synthesis, Antimicrobial Activity, and Molecular Docking Studies of Triazole-Tethered Piperazine Pharmacophores

Abstract

A series of piperazine-containing 1,2,3-triazoles were synthesized by employing the regioselective click chemistry approach using copper(I) catalyst. The synthesized compounds were evaluated for their antibacterial activity against Gram-negative (P. aeruginosa, E. coli) and Gram-positive bacteria (B. subtilis, S. aureus) and fungicidal activity against C. albicans and A. niger by resazurin-based micro-broth dilution method. Compounds 6a, 6e, 6f, and 6g exhibited significant activity compared to the standard drug ampicillin, while compounds 6f and 6g showed higher activity compared to standard drug fluconazole. Furthermore, the docking studies were performed to provide an insight into the binding mode of the most active compounds with the target proteins.