The expression and prognostic value of IFIT3 in esophageal squamous cell carcinoma.

IF 1.5 4区医学 Q4 ONCOLOGY

Translational cancer research Pub Date : 2024-11-30 Epub Date: 2024-11-01 DOI:10.21037/tcr-24-233

Jiawang Cao, Qipeng Zhang, Yiwen Xuan, Zhuan Ou, Qinghua Yu, Daoqi Zhu, Enwu Xu

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引用次数: 0

Abstract

Background: Esophageal squamous cell carcinoma (ESCC) is a malignancy for which the incidence and mortality rates are among the highest worldwide. This study aimed to look for potential biomarkers that affect the prognosis of patients with ESCC.

Methods: The target gene IFIT3 was screened through differential expression gene analysis, cluster analysis, enrichment analysis, and construction of a protein-protein interaction (PPI) network, and then validated through clinical patient tissue RNA extraction and reverse transcription quantitative polymerase chain reaction (qRT-PCR). The Mann-Whitney U test and Kaplan-Meier analysis were used to investigate the correlation between the relative expression of IFIT3 and the clinical pathological information and prognosis of ESCC patients.

Results: Gene Expression Omnibus (GEO) detected 279 differentially expressed genes (DEGs) in ESCC and paracancerous tissues. Cluster analysis and enrichment analysis showed that cluster 4 played an important role in immune-related functions. PPI network analysis showed that IFIT3 was the hub gene in cluster 4. Clinical patient tissue samples confirmed the differential expression of IFIT3 in ESCC and paracancerous tissues. Mann-Whitney U test showed that the relative expression of IFIT3 was significantly correlated with clinicopathological information in patients with ESCC. Kaplan-Meier survival analysis showed that the disease-free survival (DFS) time and overall survival (OS) time of patients with low expression of IFIT3 were significantly longer than those of patients with high expression of IFIT3, and the correlations were more significant in some subgroups. The Cox proportional hazards model showed that lymph node metastasis was an independent risk factor for the prognosis of ESCC patients.

Conclusions: IFIT3 is differentially expressed in the cancerous and paracancerous tissues of ESCC, and the relative expression level of IFIT3 is correlated with the clinical pathological characteristics and prognosis of ESCC. IFIT3 can be used as a potential biomarker for patient risk stratification and local regional metastasis in ESCC.

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IFIT3在食管鳞癌中的表达及预后价值。

背景：食管鳞状细胞癌（ESCC）是世界上发病率和死亡率最高的恶性肿瘤。本研究旨在寻找影响ESCC患者预后的潜在生物标志物。方法：通过差异表达基因分析、聚类分析、富集分析和构建蛋白-蛋白相互作用（PPI）网络筛选靶基因IFIT3，然后通过临床患者组织RNA提取和逆转录定量聚合酶链反应（qRT-PCR）进行验证。采用Mann-Whitney U检验和Kaplan-Meier分析探讨IFIT3相对表达与ESCC患者临床病理信息及预后的相关性。结果：GEO在ESCC及癌旁组织中检测到279个差异表达基因（DEGs）。聚类分析和富集分析表明，聚类4在免疫相关功能中起重要作用。PPI网络分析显示IFIT3是聚类4的枢纽基因。临床患者组织样本证实了IFIT3在ESCC和癌旁组织中的差异表达。Mann-Whitney U检验显示，IFIT3的相对表达与ESCC患者的临床病理信息显著相关。Kaplan-Meier生存分析显示，IFIT3低表达患者的无病生存期（DFS）和总生存期（OS）均明显长于IFIT3高表达患者，且在部分亚组中相关性更为显著。Cox比例风险模型显示，淋巴结转移是影响ESCC患者预后的独立危险因素。结论：IFIT3在ESCC癌组织和癌旁组织中存在差异表达，IFIT3的相对表达水平与ESCC的临床病理特征及预后相关。IFIT3可以作为ESCC患者风险分层和局部区域转移的潜在生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Translational cancer research ONCOLOGY-

CiteScore

2.10

自引率

0.00%

发文量

252

期刊介绍： Translational Cancer Research (Transl Cancer Res TCR; Print ISSN: 2218-676X; Online ISSN 2219-6803; http://tcr.amegroups.com/) is an Open Access, peer-reviewed journal, indexed in Science Citation Index Expanded (SCIE). TCR publishes laboratory studies of novel therapeutic interventions as well as clinical trials which evaluate new treatment paradigms for cancer; results of novel research investigations which bridge the laboratory and clinical settings including risk assessment, cellular and molecular characterization, prevention, detection, diagnosis and treatment of human cancers with the overall goal of improving the clinical care of cancer patients. The focus of TCR is original, peer-reviewed, science-based research that successfully advances clinical medicine toward the goal of improving patients'' quality of life. The editors and an international advisory group of scientists and clinician-scientists as well as other experts will hold TCR articles to the high-quality standards. We accept Original Articles as well as Review Articles, Editorials and Brief Articles.