Photothermal therapeutic effect by gold nanostars/extracellular vesicles nanocomplex on melanoma cells

IF 3.7 3区 医学 Q2 CHEMISTRY, MEDICINAL
Kazuki Sato, Koki Ogawa, Tatsuaki Tagami, Tetsuya Ozeki
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Abstract

Photothermal therapy (PTT) is a method for treating cancer using the heat generated by light irradiation, often in combination with light-absorbing materials. Efficient PTT requires a drug delivery system to deliver light-absorbing materials to cancerous tissues. Gold nanostars (GNSs) enable efficient PTT through absorbing long-wavelength light. In this study, we established a platform for preparing GNSs-loaded extracellular vesicles (EVs), that were expected to provide the combined biological functionality of EVs and photothermal conversion properties. By electrostatically binding cationic polyethylene glycol (PEG)-modified GNSs (PEG-GNSs) to naturally negatively charged EVs, EV/PEG-GNSs nanocomplexes (EV-GNSs) were obtained by simply mixing the two components. The prepared EV-GNSs were approximately 200 nm in size and exhibited a negative surface charge. The surface protein marker of EVs, CD63, was detected using western blot, suggesting that the EVs were intact. In vitro evaluation showed that EV-GNSs exhibited better photothermal conversion properties than PEG-GNSs alone. When EV-GNSs were added to the cells, high laser-responsive cytotoxicity was observed following laser irradiation. Thus, EV-GNSs developed in this study may be suitable for PTT using gold-based nanoparticles.

Abstract Image

纳米金纳米星/细胞外囊泡纳米复合物对黑色素瘤细胞的光热治疗作用。
光热疗法(PTT)是一种利用光照射产生的热量治疗癌症的方法,通常与吸光材料结合使用。有效的PTT需要一个药物输送系统将吸收光的材料输送到癌组织。金纳米星(GNSs)通过吸收长波长的光来实现高效的PTT。在这项研究中,我们建立了一个制备gns负载的细胞外囊泡(ev)的平台,该平台有望提供ev的生物功能和光热转换特性。将阳离子聚乙二醇(PEG)修饰的GNSs (PEG-GNSs)与自然带负电的电动汽车静电结合,简单混合得到EV/PEG- gns纳米配合物(EV-GNSs)。制备的EV-GNSs尺寸约为200 nm,表面带负电荷。western blot检测EVs表面蛋白标记CD63,表明EVs是完整的。体外评价表明,EV-GNSs的光热转换性能优于单独的PEG-GNSs。将EV-GNSs添加到细胞中,观察到激光照射后的高激光响应性细胞毒性。因此,本研究开发的EV-GNSs可能适用于使用金基纳米颗粒的PTT。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.30
自引率
13.20%
发文量
367
审稿时长
33 days
期刊介绍: The Journal of Pharmaceutical Sciences will publish original research papers, original research notes, invited topical reviews (including Minireviews), and editorial commentary and news. The area of focus shall be concepts in basic pharmaceutical science and such topics as chemical processing of pharmaceuticals, including crystallization, lyophilization, chemical stability of drugs, pharmacokinetics, biopharmaceutics, pharmacodynamics, pro-drug developments, metabolic disposition of bioactive agents, dosage form design, protein-peptide chemistry and biotechnology specifically as these relate to pharmaceutical technology, and targeted drug delivery.
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