Hu Qiu , Lijuan Gao , Wei Shi , Jing Wang , Bin Li , Shaobo Ke , Jiamei Chen , Yi Gong , Yong Wu , Wensi Zhao , Yongshun Chen
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引用次数: 0
Abstract
Elaiophylin is known to exert antitumor effects through certain signaling pathways; however, no reports regarding its effects on esophageal cancer are available. This study explored the effects of elaiophylin in esophageal squamous cell carcinoma (ESCC) cells. Transwell and immunofluorescence assays confirmed that elaiophylin inhibited the migration and proliferation of ESCC cells, and western blotting assays showed that it affected apoptosis-related gene expression in ESCC cells. Based on RNA-seq analyses, Single-cell RNA-seq, a human cancer pathway phosphorylation antibody array, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomics analyses, we found that elaiophylin was related to low expression of EIF4B and activation of the phosphoinositide 3-kinase (PI3K)/AKT signaling pathway. In both in vitro and in vivo experiments, ESCC cells treated with elaiophylin showed low EIF4B expression, which inhibited their proliferation and promoted apoptosis by activating the PI3K/AKT signaling pathway; EIF4B overexpression could reverse these effects of elaiophylin on ESCC cells. Therefore, our results indicate that elaiophylin targets EIF4B to inhibit ESCC cell proliferation via the PI3K/AKT signaling pathway. Targeting elaiophylin or the EIF4B/PI3K/AKT signaling pathway may produce new methods for ESCC treatment.
Elaiophylin通过某些信号通路发挥抗肿瘤作用;然而,尚无关于其对食管癌影响的报道。本研究探讨松茶碱在食管鳞状细胞癌(ESCC)细胞中的作用。Transwell和免疫荧光实验证实,elaiophylin抑制ESCC细胞的迁移和增殖,western blotting实验显示,elaiophylin影响ESCC细胞凋亡相关基因的表达。基于RNA-seq、单细胞RNA-seq、人类癌症通路磷酸化抗体阵列、Gene Ontology和Kyoto Encyclopedia of Genes and Genomics分析,我们发现elaiophylin与EIF4B的低表达和PI3K /AKT信号通路的激活有关。在体外和体内实验中,elaiophylin处理的ESCC细胞EIF4B表达较低,通过激活PI3K/AKT信号通路抑制细胞增殖,促进细胞凋亡;EIF4B过表达可逆转松茶碱对ESCC细胞的这些作用。因此,我们的研究结果表明,elaiophylin通过PI3K/AKT信号通路靶向EIF4B抑制ESCC细胞增殖。靶向elaiophylin或EIF4B/PI3K/AKT信号通路可能为ESCC治疗提供新的方法。
期刊介绍:
Cancer Letters is a reputable international journal that serves as a platform for significant and original contributions in cancer research. The journal welcomes both full-length articles and Mini Reviews in the wide-ranging field of basic and translational oncology. Furthermore, it frequently presents Special Issues that shed light on current and topical areas in cancer research.
Cancer Letters is highly interested in various fundamental aspects that can cater to a diverse readership. These areas include the molecular genetics and cell biology of cancer, radiation biology, molecular pathology, hormones and cancer, viral oncology, metastasis, and chemoprevention. The journal actively focuses on experimental therapeutics, particularly the advancement of targeted therapies for personalized cancer medicine, such as metronomic chemotherapy.
By publishing groundbreaking research and promoting advancements in cancer treatments, Cancer Letters aims to actively contribute to the fight against cancer and the improvement of patient outcomes.