Cyclophosphamide-induced multiple organ dysfunctions: unravelling of dose dependent toxic impact on biochemistry and histology.

IF 2.2 4区医学 Q3 TOXICOLOGY

Toxicology Research Pub Date : 2024-12-17 eCollection Date: 2024-12-01 DOI:10.1093/toxres/tfae201

Asim Amitabh Sahu, Ankita Mukherjee, Satendra Kumar Nirala, Monika Bhadauria

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Abstract

Background: Cyclophosphamide, an immunosuppressive alkylating agent, has been used against breast cancer, lymphoma and myeloid leukemia. Despite various therapeutic uses, its toxic impacts on multiple organs remains to be fully elucidated.

Aim: This study aimed to investigate dose dependent toxic impact of cyclophosphamide on liver, kidney, brain and testis emphasizing serum and tissue biochemical and histological alterations.

Materials and methods: Experimental design consisted of five groups of albino rats. Group 1-5 were administered vehicle for five consecutive days. On 6^th day, group 1 received vehicle only and termed as control; group 2-5 received cyclophosphamide through intraperitoneal route at the rate of 50, 100, 150 and 200 mg/kg dose, respectively. After 24 h of the last administration, rats were euthanised; serum and tissue biochemistry; histology, sperm count and its motility were performed.

Results: Serological, biochemical and histological indices exhibited dose dependent deviations from their regular status as a marker of toxicity in liver, kidney, brain and testis. Tukey's HSD post hoc test revealed maximum damage in multiple organs with 200 mg/kg dose of cyclophosphamide.

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环磷酰胺诱导的多器官功能障碍：剂量依赖性毒性对生物化学和组织学影响的揭示。

背景：环磷酰胺是一种免疫抑制烷基化剂，已被用于治疗乳腺癌、淋巴瘤和髓性白血病。尽管有多种治疗用途，但其对多个器官的毒性影响仍有待充分阐明。目的：研究环磷酰胺对大鼠肝、肾、脑和睾丸的剂量依赖性毒性作用，重点观察血清和组织生化及组织学改变。材料与方法：实验设计为5组白化大鼠。1 ~ 5组连续给药5 d。第6天，组1只给药，称为对照组；2 ~ 5组腹腔注射环磷酰胺，剂量分别为50、100、150、200 mg/kg。末次给药24 h后，对大鼠实施安乐死；血清及组织生化；观察组织学、精子计数和精子活力。结果：血清学、生化和组织学指标作为肝、肾、脑和睾丸的毒性标志物，表现出剂量依赖性偏差。Tukey的HSD事后试验显示，200 mg/kg剂量的环磷酰胺对多个器官的损害最大。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicology Research TOXICOLOGY-

CiteScore

3.60

自引率

0.00%

发文量

期刊介绍： A multi-disciplinary journal covering the best research in both fundamental and applied aspects of toxicology