Yan Zhang, Yisi Yang*, Tianqing Liu, Dan Dai, Linqing Zhou, Wei Liu and Kuai Zhou,
{"title":"Laboratory Innovation Project of Substance Separation and Detection Using Self-Made Low-Pressure Liquid Chromatography","authors":"Yan Zhang, Yisi Yang*, Tianqing Liu, Dan Dai, Linqing Zhou, Wei Liu and Kuai Zhou, ","doi":"10.1021/acs.jchemed.4c0093410.1021/acs.jchemed.4c00934","DOIUrl":null,"url":null,"abstract":"<p >The chromatography method is deeply used in separation and analysis of organic compounds, and it is also an important element in organic chemistry experiment or advanced experiment courses. However, in undergraduate teaching laboratories, the number of liquid chromatography instruments is relatively limited, and most of the components of the instrument are enclosed in a box, which can not meet the learning and operating needs of all students. Thus, in our project, self-made low pressure liquid chromatography (SLLC) was designed and applied in an innovative experimental project to make sure that every student could build the device independently and use it to separate and detect chlorophyll and colorless conjugated organic mixtures. Gratifyingly, all students were interested in the experimental content. With the aids of hands-on activities and visually intuitive phenomena, the structure, principles, and operation of liquid chromatography (LC) can be fully understood and mastered. The project is suitable for promotion and practice in universities at all levels because it only took four credit hours and about 100 RMB (about 13 dollars) on the self-made SLLC.</p>","PeriodicalId":43,"journal":{"name":"Journal of Chemical Education","volume":"101 12","pages":"5345–5352 5345–5352"},"PeriodicalIF":2.5000,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Chemical Education","FirstCategoryId":"92","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.jchemed.4c00934","RegionNum":3,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
The chromatography method is deeply used in separation and analysis of organic compounds, and it is also an important element in organic chemistry experiment or advanced experiment courses. However, in undergraduate teaching laboratories, the number of liquid chromatography instruments is relatively limited, and most of the components of the instrument are enclosed in a box, which can not meet the learning and operating needs of all students. Thus, in our project, self-made low pressure liquid chromatography (SLLC) was designed and applied in an innovative experimental project to make sure that every student could build the device independently and use it to separate and detect chlorophyll and colorless conjugated organic mixtures. Gratifyingly, all students were interested in the experimental content. With the aids of hands-on activities and visually intuitive phenomena, the structure, principles, and operation of liquid chromatography (LC) can be fully understood and mastered. The project is suitable for promotion and practice in universities at all levels because it only took four credit hours and about 100 RMB (about 13 dollars) on the self-made SLLC.
期刊介绍:
The Journal of Chemical Education is the official journal of the Division of Chemical Education of the American Chemical Society, co-published with the American Chemical Society Publications Division. Launched in 1924, the Journal of Chemical Education is the world’s premier chemical education journal. The Journal publishes peer-reviewed articles and related information as a resource to those in the field of chemical education and to those institutions that serve them. JCE typically addresses chemical content, activities, laboratory experiments, instructional methods, and pedagogies. The Journal serves as a means of communication among people across the world who are interested in the teaching and learning of chemistry. This includes instructors of chemistry from middle school through graduate school, professional staff who support these teaching activities, as well as some scientists in commerce, industry, and government.