Plasma CircCYP24A1 as a Novel Biomarker of Esophageal Squamous Cell Carcinoma.

IF 2.7 4区医学 Q3 ONCOLOGY

Technology in Cancer Research & Treatment Pub Date : 2024-01-01 DOI:10.1177/15330338241295313

Ruolan Zhang, Jianlin Liu, Hang Yang, Jinsong Tan, Rong Xiong, Yun Liu, Gang Feng, Guiqin Song, Kang Liu

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引用次数: 0

Abstract

Background: A clinical challenge in esophageal squamous cell carcinoma (ESCC) remains the lack of applicable plasma biomarkers for screening and diagnosis. Circular RNAs (circRNAs) hold great potential as biomarkers for cancer. The study aims to explore a circRNA as a potential plasma biomarker for screening strategies and diagnostic approaches to ESCC.

Methods: Upregulated circRNAs were identified through RNA sequencing, with circCYP24A1 being identified as the target circRNA. Fluorescence in situ hybridization was employed to detect the expression of circCYP24A1 in ESCC tissue microarrays, aiming to assess the expression of circCYP24A1 in a large population and its correlation with clinical indicators. Subsequently, qRT-PCR analysis was performed on plasma samples from both ESCC patients and healthy controls to evaluate the expression levels of circCYP24A1, exploring its potential as a biomarker. Finally, the functions of circCYP24A1 were validated through CCK-8 assay, wound healing assay, trans-well assays and western blot assays.

Results: CircCYP24A1 demonstrated upregulation in both plasma and tissues, exhibiting correlations with lymph node metastasis, TNM staging, and prognosis in ESCC. The circCYP24A1 achieved a perfect area under the curve of 0.94 for the diagnosis of ESCC, and an area under the curve of 0.76 for the prediction of lymph node metastasis. Furthermore, functional loss assays revealed that circCYP24A1 effectively promotes the epithelial-mesenchymal transition and tumor metastasis in vitro.

Conclusions: CircCYP24A1 emerges as a potential plasma diagnostic biomarker and a predictive factor for LNM for ESCC.

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血浆CircCYP24A1作为食管鳞状细胞癌的新生物标志物

背景：食管鳞状细胞癌（ESCC）的临床难题仍然是缺乏可用于筛查和诊断的血浆生物标志物。环状 RNA（circRNA）作为癌症生物标志物具有巨大潜力。本研究旨在探索一种circRNA作为潜在的血浆生物标志物，用于ESCC的筛查策略和诊断方法：方法：通过RNA测序确定了上调的circRNA，其中circCYP24A1被确定为目标circRNA。采用荧光原位杂交技术检测 ESCC 组织芯片中 circCYP24A1 的表达，旨在评估大量人群中 circCYP24A1 的表达及其与临床指标的相关性。随后，对 ESCC 患者和健康对照者的血浆样本进行了 qRT-PCR 分析，以评估 circCYP24A1 的表达水平，探索其作为生物标志物的潜力。最后，通过CCK-8试验、伤口愈合试验、转孔试验和Western印迹试验验证了circCYP24A1的功能：结果：circCYP24A1在血浆和组织中均有上调，与ESCC的淋巴结转移、TNM分期和预后相关。circCYP24A1 诊断 ESCC 的曲线下面积为 0.94，预测淋巴结转移的曲线下面积为 0.76。此外，功能丧失试验显示，circCYP24A1能有效促进上皮-间质转化和体外肿瘤转移：CircCYP24A1是一种潜在的血浆诊断生物标记物，也是ESCC淋巴结转移的预测因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Technology in Cancer Research & Treatment 医学-肿瘤学

CiteScore

4.40

自引率

0.00%

发文量

202

审稿时长

2 months

期刊介绍： Technology in Cancer Research & Treatment (TCRT) is a JCR-ranked, broad-spectrum, open access, peer-reviewed publication whose aim is to provide researchers and clinicians with a platform to share and discuss developments in the prevention, diagnosis, treatment, and monitoring of cancer.