Screening study of hydroxytyrosol metabolites from in vitro fecal fermentation and their interaction with intestinal barrier repair receptor AhR

IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY
Yuqing Song, Mengting Li, Jingle Liu, Juan Wang, Aimei Zhou, Yong Cao, Shan Duan, Qun Wang
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Abstract

Olive oil polyphenol hydroxytyrosol (HT) significantly repairs intestinal barrier function, but its absorption in the stomach and small intestine is limited. The metabolites of unabsorbed HT that reach the colon are crucial, yet their effects on colonic microbiota and intestinal barrier repair remain unclear. This study utilized in vitro simulated digestion and colonic fecal fermentation to investigate HT's digestion and fermentation. Results indicated that 79.25% of HT potentially reached the colon intact. Further 16S rDNA, targeted, and untargeted metabolomics analyses showed that HT can be decomposed by colonic microbiota, producing aromatic hydrocarbon metabolites and regulating gut microbiota structure. It promotes the growth of gut microbiota, such as Bacteroides, Faecalibacterium, Klebsiella, and Lachnospira, which degrade HT. Additionally, HT's intervention conversely affected the production of tryptophan-derived metabolites and short-chain fatty acids (SCFAs). Subsequently, computer-simulated molecular docking technology was used to simulate the binding affinity between HT metabolites and derived metabolites and the intestinal barrier repair-related receptor aryl hydrocarbon receptor (AhR). Indole-3-acetic acid, indole-3-acetaldehyde, skatole, kynurenine, and homovanillic acid could tightly bind to the amino acid residues of the AhR receptor, with binding energies all ˂−6.0 kcal/mol, suggesting that these metabolites may enhance the intestinal barrier function through the AhR signaling pathway.

Abstract Image

体外粪便发酵羟酪醇代谢物筛选及其与肠道屏障修复受体AhR相互作用的研究。
橄榄油多酚羟基酪醇(HT)可显著修复肠道屏障功能,但其在胃和小肠中的吸收有限。未吸收的羟色胺到达结肠的代谢物是至关重要的,但它们对结肠微生物群和肠屏障修复的影响尚不清楚。本研究采用体外模拟消化和结肠粪便发酵的方法对HT的消化和发酵进行了研究。结果显示79.25%的HT可能完好无损地到达结肠。进一步的16S rDNA、靶向和非靶向代谢组学分析表明,HT可以被结肠微生物群分解,产生芳香烃代谢物并调节肠道微生物群结构。它促进肠道微生物群的生长,如拟杆菌、粪杆菌、克雷伯氏菌和毛螺旋体,它们可以降解HT。此外,HT的干预反过来影响了色氨酸衍生代谢物和短链脂肪酸(SCFAs)的产生。随后,采用计算机模拟分子对接技术模拟HT代谢物及衍生代谢物与肠道屏障修复相关受体芳烃受体(aryl hydrocarbon receptor, AhR)的结合亲和力。吲哚-3-乙酸、吲哚-3-乙醛、粪甲素、犬尿氨酸和同型香草酸能与AhR受体氨基酸残基紧密结合,结合能均小于-6.0 kcal/mol,表明这些代谢物可能通过AhR信号通路增强肠道屏障功能。
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来源期刊
Journal of Food Science
Journal of Food Science 工程技术-食品科技
CiteScore
7.10
自引率
2.60%
发文量
412
审稿时长
3.1 months
期刊介绍: The goal of the Journal of Food Science is to offer scientists, researchers, and other food professionals the opportunity to share knowledge of scientific advancements in the myriad disciplines affecting their work, through a respected peer-reviewed publication. The Journal of Food Science serves as an international forum for vital research and developments in food science. The range of topics covered in the journal include: -Concise Reviews and Hypotheses in Food Science -New Horizons in Food Research -Integrated Food Science -Food Chemistry -Food Engineering, Materials Science, and Nanotechnology -Food Microbiology and Safety -Sensory and Consumer Sciences -Health, Nutrition, and Food -Toxicology and Chemical Food Safety The Journal of Food Science publishes peer-reviewed articles that cover all aspects of food science, including safety and nutrition. Reviews should be 15 to 50 typewritten pages (including tables, figures, and references), should provide in-depth coverage of a narrowly defined topic, and should embody careful evaluation (weaknesses, strengths, explanation of discrepancies in results among similar studies) of all pertinent studies, so that insightful interpretations and conclusions can be presented. Hypothesis papers are especially appropriate in pioneering areas of research or important areas that are afflicted by scientific controversy.
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