Production of Sea Buckthorn Oil via Supercritical Carbon Dioxide Extraction of Sea Buckthorn Fruit Pressed Cake with Subsequent Centrifugation

Abstract

A simple method for obtaining sea buckthorn (Hyppophae rhamnoides) oil with carotenoid content high enough to comply with the requirements for sea buckthorn oil based wound remedy is proposed. A method is based on supercritical carbon dioxide extraction of dried pressed cake of sea buckthorn berries at high extraction pressures (up to 55 MPa) for obtaining higher carotenoid concentration and subsequent centrifugation in order to separate solid wax fraction. Chromatography and spectrophotometry analyses of such oil support the potential perspective of the proposed method.