Two-photon brightness of NIR-emitting, atomically precise DNA-stabilized silver nanoclusters

IF 7.6 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Chemical Science Pub Date : 2024-12-16 DOI:10.1039/d4sc05853d

Joanna Olesiak-Banska, Agata Hajda, Rweetuparna Guha, Stacy M. Copp

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引用次数: 0

Abstract

Near-infrared (NIR) emitters with high two-photon absorption (2PA) cross-sections are of interest to enable in vivo imaging in the tissue transparency windows. This study explores the potential of DNA-stabilized silver nanoclusters (AgN-DNAs) as water-soluble two-photon absorbers. We investigate 2PA of four different atomically precise AgN-DNA species with far-red to NIR emission and varying nanocluster and ligand compositions. 2PA cross-sections, σ2, were determined by two-photon excited luminescence (TPEL) technique for a wide wavelength range from 810 to 1400 nm. The AgN-DNAs exhibited reversed strength of corresponding transitions in the two-photon regime, as compared to one-photon, which further demonstrates the complex photophysics of these emitters. Maximal 2PA cross-section value (813 GM) was observed for (DNA)3[Ag21]15+, which is stabilized by 3 DNA oligomers. (DNA)2[Ag16Cl2]8+ presented distinct 2PA behavior from the AgN-DNAs without chlorido ligands, with a high 2PA of 176 GM at 1050 nm. Our findings support the potential of AgN-DNAs as NIR-to-NIR two-photon probes that are both excited and emit in the NIR. Their high σ2 and fluorescence quantum yield values result in superior two-photon brightness on the order of ~102 GM, significantly higher than water-soluble organic fluorophores.

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来源期刊

Chemical Science CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

14.40

自引率

4.80%

发文量

1352

审稿时长

2.1 months

期刊介绍： Chemical Science is a journal that encompasses various disciplines within the chemical sciences. Its scope includes publishing ground-breaking research with significant implications for its respective field, as well as appealing to a wider audience in related areas. To be considered for publication, articles must showcase innovative and original advances in their field of study and be presented in a manner that is understandable to scientists from diverse backgrounds. However, the journal generally does not publish highly specialized research.