Zonal turnover of cell poles of Bacillus subtilis

Abstract

Turnover of cell walls of Bacillus subtilis occurs in three distinct phases: a lag phase, a relatively rapid phase persisting for 2–3 generations and a much slower phase continuing for several additional generations. A lectin probe revealed that cell pole material was lost during the slow phase of turnover and that the loss of wall occurred in zones, beginning at the cylinder-pole junction and continuing to the cell tip. This is in contrast to cell wall turnover in cylinders where turnover occurs randomly at many surface sites.