mRNA m6A detection

IF 50.1 Q1 MULTIDISCIPLINARY SCIENCES

Nature reviews. Methods primers Pub Date : 2024-12-12 DOI:10.1038/s43586-024-00365-9

Sharon Moshitch-Moshkovitz, Michal Sevilla-Sharon, Reut Ashwal-Fluss, Efrat Glick-Saar, Gideon Rechavi, Dan Dominissini

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Abstract

N6-methyladenosine (m6A) is the most prevalent internal mRNA modification. Recent research has highlighted its role as a key regulator of gene expression, influencing cellular processes and determining cell fate. Advances in techniques for global mapping of m6A, the discovery of m6A demethylases that enhance its dynamic properties and the identification of reader proteins that interact with m6A have substantially propelled this field forward. This Primer outlines the available tools for detecting and mapping m6A, discusses the strengths and limitations of each method and offers guidance on selecting the most suitable approach. Identifying and detecting m6A lays the groundwork for functional studies that address important biological and medical questions. N6-methyladenosine (m6A) is an mRNA modification influencing gene expression. Advanced methodologies for mapping m6A enhance understanding of its dynamic roles and interactions. In this Primer, Moshitch-Moshkovitz et al. discuss various detection tools and analysis techniques facilitating insights into RNA epitranscriptomics.

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mRNA m6A检测

n6 -甲基腺苷（m6A）是最常见的内部mRNA修饰。最近的研究强调了它作为基因表达的关键调节剂，影响细胞过程和决定细胞命运的作用。m6A全局定位技术的进步，增强其动态特性的m6A去甲基化酶的发现，以及与m6A相互作用的解读蛋白的鉴定，极大地推动了这一领域的发展。本入门概述了用于检测和映射m6A的可用工具，讨论了每种方法的优势和局限性，并提供了选择最合适方法的指导。识别和检测m6A为解决重要的生物学和医学问题的功能研究奠定了基础。n6 -甲基腺苷（m6A）是一种影响基因表达的mRNA修饰。用于映射m6A的高级方法增强了对其动态角色和相互作用的理解。在本引物中，Moshitch-Moshkovitz等人讨论了各种检测工具和分析技术，有助于深入了解RNA表转录组学。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature reviews. Methods primers

CiteScore

46.10

自引率

0.00%

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