{"title":"Exploring the promising potential of alcohol extract from the aerial part of dill in ameliorating DSS-induced ulcerative colitis in mice.","authors":"Chen-Huan Qiao, Tian-Tian Liu, Yao-Yao Li, Shi-Dan Wang, Yu-Xin Chen","doi":"10.1016/j.jep.2024.119237","DOIUrl":null,"url":null,"abstract":"<p><strong>Ethnopharmacological relevance: </strong>Dill (Anethum graveolens L.) is a typical Uyghur medicine. It is traditionally used to treat sticky and stagnant dampness, hiccups and food stagnation, intestinal obstruction, and anorectal diseases.</p><p><strong>Study objective: </strong>Our study is designed to investigate the potential of alcohol extract from the aerial part of dill in ameliorating ulcerative colitis induced by Dextran Sulfate Sodium Salt (DSS) in mice.</p><p><strong>Materials and methods: </strong>In this paper, the chemical composition of the aerial part of dill was speculated from the data obtained by LC-MS and determined by comparing with 10 standards through HPLC. The aerial part of fresh dill was dried, crushed, sieved, and then extracted with 70% ethanol to obtain DE. The lipopolysaccharide (LPS)-induced RAW264.7 cells were used to test the anti-inflammatory activity of DE in vitro. The impact of DE on UC was also studied in vivo. UC was induced by drinking 2.5% DSS to C57BL/6 mice for 6 days. The positive control group received 5-aminosalicylic acid (5-ASA) by gavage, and the low and high-dose treatment groups were respectively given 200 mg/kg and 400 mg/kg of DE by gavage daily for 7 days from the first day.</p><p><strong>Results: </strong>DE significantly reduces the disease activity index (DAI) and colon histopathological damage. DE can also alleviate oxidative stress and inflammation in UC mice by reducing IL-6, IL-1β, MDA, and MPO levels and increasing CAT and GSH levels in colonic tissues. DE can protect the integrity of the colonic mucosal barrier by reducing damage to goblet cells, increasing the levels of mucin MUC2, and regulating the expression of tight junction proteins such as ZO-1, Occludin, Claudin-1, and Claudin-2. In addition, DE improves the ratio of beneficial and harmful bacteria, thus further alleviating the imbalance of intestinal flora.</p><p><strong>Conclusion: </strong>DE has anti-inflammatory activity in vitro and an ameliorative effect on DSS-induced UC in mice by alleviating oxidative stress and inflammation, protecting the integrity of the intestinal barrier, and regulating intestinal flora.</p>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":" ","pages":"119237"},"PeriodicalIF":4.8000,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of ethnopharmacology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.jep.2024.119237","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
引用次数: 0
Abstract
Ethnopharmacological relevance: Dill (Anethum graveolens L.) is a typical Uyghur medicine. It is traditionally used to treat sticky and stagnant dampness, hiccups and food stagnation, intestinal obstruction, and anorectal diseases.
Study objective: Our study is designed to investigate the potential of alcohol extract from the aerial part of dill in ameliorating ulcerative colitis induced by Dextran Sulfate Sodium Salt (DSS) in mice.
Materials and methods: In this paper, the chemical composition of the aerial part of dill was speculated from the data obtained by LC-MS and determined by comparing with 10 standards through HPLC. The aerial part of fresh dill was dried, crushed, sieved, and then extracted with 70% ethanol to obtain DE. The lipopolysaccharide (LPS)-induced RAW264.7 cells were used to test the anti-inflammatory activity of DE in vitro. The impact of DE on UC was also studied in vivo. UC was induced by drinking 2.5% DSS to C57BL/6 mice for 6 days. The positive control group received 5-aminosalicylic acid (5-ASA) by gavage, and the low and high-dose treatment groups were respectively given 200 mg/kg and 400 mg/kg of DE by gavage daily for 7 days from the first day.
Results: DE significantly reduces the disease activity index (DAI) and colon histopathological damage. DE can also alleviate oxidative stress and inflammation in UC mice by reducing IL-6, IL-1β, MDA, and MPO levels and increasing CAT and GSH levels in colonic tissues. DE can protect the integrity of the colonic mucosal barrier by reducing damage to goblet cells, increasing the levels of mucin MUC2, and regulating the expression of tight junction proteins such as ZO-1, Occludin, Claudin-1, and Claudin-2. In addition, DE improves the ratio of beneficial and harmful bacteria, thus further alleviating the imbalance of intestinal flora.
Conclusion: DE has anti-inflammatory activity in vitro and an ameliorative effect on DSS-induced UC in mice by alleviating oxidative stress and inflammation, protecting the integrity of the intestinal barrier, and regulating intestinal flora.
期刊介绍:
The Journal of Ethnopharmacology is dedicated to the exchange of information and understandings about people''s use of plants, fungi, animals, microorganisms and minerals and their biological and pharmacological effects based on the principles established through international conventions. Early people confronted with illness and disease, discovered a wealth of useful therapeutic agents in the plant and animal kingdoms. The empirical knowledge of these medicinal substances and their toxic potential was passed on by oral tradition and sometimes recorded in herbals and other texts on materia medica. Many valuable drugs of today (e.g., atropine, ephedrine, tubocurarine, digoxin, reserpine) came into use through the study of indigenous remedies. Chemists continue to use plant-derived drugs (e.g., morphine, taxol, physostigmine, quinidine, emetine) as prototypes in their attempts to develop more effective and less toxic medicinals.