Invasive Plants' Root Extracts Display Stronger Allelopathic Activity on the Germination and Seedling Growth of a New Zealand Native Species than Extracts of Another Native Plant or Conspecifics.

Abstract

The release of allelochemicals is one of the contributing factors to the success of invasive plants in their non-native ranges. It has been hypothesised that the impact of chemicals released by a plant on its neighbours is shaped by shared coevolutionary history, making natives more susceptible to "new" chemicals released by introduced plant species (novel weapons hypothesis). We explored this hypothesis in a New Zealand system where the two invasive plants of European origin, Cytisus scoparius (Scotch broom) and Calluna vulgaris (heather) cooccur with natives like Chionochloa rubra (red tussock) and Leptospermum scoparium (mānuka). We characterised the chemical composition of root extracts of broom, heather, red tussock and mānuka using gas chromatography-mass spectrometry and then investigated the influence of aqueous root extracts at different concentrations (0.1%, 1%, 5%, 50% and 100% v/v) on mānuka seed germination and seedling growth (root and shoot length and biomass), using deionised water as control. The results show clear distinctions in the chemical composition of the four plants' root extracts, with 4-O-methylmannose dominating the broom extract and (E)-pinocarveol the heather extract, while 16-kaurene and methyl palmitate were abundant in both mānuka and tussock extracts. We found a significant effect of invasive plant (heather and broom) root extracts on mānuka germination at all concentrations tested, and adverse effects on seedling growth and biomass only at higher concentrations (≥ 5%). Broom displayed stronger allelopathic effects than heather at the highest concentration (100%). For extracts of conspecific and other native species (mānuka and red tussock) allelopathic effects were only observed at very high concentrations (50 and 100%) and were generally weaker than those observed for invasive plants. These results show that while both native and invasive plants produce chemicals with allelopathic potential, native species are likely to be more vulnerable to the allelopathic effects of species they did not co-evolve with, supporting the novel weapons hypothesis. However, this study also highlights differences in allelopathic potential between invasive species.