Fetuin-A as a plausible biomarker for cardiac valve calcification in rheumatic heart disease patients from North India.

IF 0.7 Q3 Medicine

ASIAN CARDIOVASCULAR & THORACIC ANNALS Pub Date : 2024-12-12 DOI:10.1177/02184923241306482

Aishwarya Rani, Lakhwinder Singh, Anuradha Chakraborti, Sameer Gupta, Harkant Singh, Devinder Toor

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引用次数: 0

Abstract

Background: Rheumatic heart disease (RHD) remains a persistent public health challenge, particularly prevalent in developing and underdeveloped regions despite concerted global eradication efforts. The progressive stage of RHD, marked by valvular calcification, necessitates the imperative need to identify prognostic biomarkers. Fetuin-A, well-known for its role as a negative inhibitor of ectopic calcification, is investigated in our study as a potential biomarker for cardiac valve calcification in RHD patients.

Methods: Individuals with confirmed presence of RHD via echocardiography, who exhibited moderate to severe cardiac valve involvement, were enrolled alongside age and sex-matched healthy controls. Enzyme Linked Immunosorbent Assay was performed to analyse serum concentration of fetuin-A in healthy controls and RHD patients. Cytokine profiling was carried out using Cytometric Bead Array.

Results: Sixty confirmed RHD patients along with age and sex-matched case-control were evaluated for their serum fetuin-A and serum cytokines levels. Our findings reveal significantly reduced serum fetuin-A levels in RHD patients (1.96 ± 0.608 ng/ml) compared to healthy controls (2.85 ± 0.55 ng/ml) (p < 0.0001). Cytokine profiling shows nearly two-fold elevations in interleukin (IL)-17A, tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), IL-6, IL-10, IL-4 and IL-2 levels among RHD patients versus healthy controls, with IL-6 showing the maximum elevation in serum concentration. Pearson's correlation coefficient (r) values indicate a strong negative correlation between fetuin-A levels in RHD patients and various inflammatory cytokines, including IL-17A (-0.9754), IFN-γ (-0.8142), TNF-α (-0.8281), IL-10 (-0.9183), IL-6 (-0.8479), IL-4 (-0.9182) and IL-2 (-0.9430) (p < 0.05, statistically significant).

Conclusion: This study suggests that fetuin-A can be explored as a plausible biomarker for cardiac valve calcification and has an inverse correlation with inflammatory cytokines involved in RHD.

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胎儿素a可能是北印度风湿性心脏病患者心脏瓣膜钙化的生物标志物

背景：风湿性心脏病（RHD）仍然是一个持续的公共卫生挑战，尽管全球一致努力根除，但在发展中国家和不发达地区尤其普遍。RHD的进展阶段，以瓣膜钙化为标志，迫切需要确定预后生物标志物。Fetuin-A因其作为异位钙化的负抑制剂而闻名，在我们的研究中被研究为RHD患者心脏瓣膜钙化的潜在生物标志物。方法：通过超声心动图确认存在RHD的个体，表现出中度至重度心脏瓣膜受累，与年龄和性别匹配的健康对照组一起入组。采用酶联免疫吸附法分析健康对照和RHD患者血清中胎儿素a的浓度。细胞因子谱分析采用细胞细胞阵列技术。结果：60例确诊的RHD患者以及年龄和性别匹配的病例对照评估了他们的血清胎儿素a和血清细胞因子水平。结果显示，RHD患者血清胎儿素a水平（1.96±0.608 ng/ml）显著低于健康对照组（2.85±0.55 ng/ml）（p r），表明RHD患者血清胎儿素a水平与多种炎症因子IL-17A（-0.9754）、IFN-γ(-0.8142)、TNF-α(-0.8281)、IL-10（-0.9183）、IL-6（-0.8479）、IL-4（-0.9182）、IL-2（-0.9430）呈显著负相关(p)。这项研究表明，胎儿素a可以作为心脏瓣膜钙化的一种合理的生物标志物，并且与RHD相关的炎症细胞因子呈负相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ASIAN CARDIOVASCULAR & THORACIC ANNALS Multiple-

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： The Asian Cardiovascular and Thoracic Annals is an international peer-reviewed journal pertaining to cardiovascular and thoracic medicine. Besides original clinical manuscripts, we welcome research reports, product reviews, reports of new techniques, and findings of special significance to Asia and the Pacific Rim. Case studies that have significant novel original observations, are instructive, include adequate methodological details and provide conclusions. Workshop proceedings, meetings and book reviews, letters to the editor, and meeting announcements are encouraged along with relevant articles from authors.