Therapeutic effects of low-intensity transcranial focused ultrasound stimulation on ischemic stroke in rats: An in vivo evaluation using electrical impedance tomography.

IF 5.9 2区 医学 Q2 CELL BIOLOGY
Neural Regeneration Research Pub Date : 2026-03-01 Epub Date: 2024-12-07 DOI:10.4103/NRR.NRR-D-24-00128
Jiecheng Guo, Sixuan He, Li Yan, Lei Wang, Xuetao Shi, Huijing Hu, Le Li
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引用次数: 0

Abstract

JOURNAL/nrgr/04.03/01300535-202603000-00042/figure1/v/2025-06-16T082406Z/r/image-tiff Although previous studies have demonstrated that transcranial focused ultrasound stimulation protects the ischemic brain, clear criteria for the stimulation time window and intensity are lacking. Electrical impedance tomography enables real-time monitoring of changes in cerebral blood perfusion within the ischemic brain, but investigating the feasibility of using this method to assess post-stroke rehabilitation in vivo remains critical. In this study, ischemic stroke was induced in rats through middle cerebral artery occlusion surgery. Transcranial focused ultrasound stimulation was used to treat the rat model of ischemia, and electrical impedance tomography was used to measure impedance during both the acute stage of ischemia and the rehabilitation stage following the stimulation. Electrical impedance tomography results indicated that cerebral impedance increased after the onset of ischemia and decreased following transcranial focused ultrasound stimulation. Furthermore, the stimulation promoted motor function recovery, reduced cerebral infarction volume in the rat model of ischemic stroke, and induced the expression of brain-derived neurotrophic factor in the ischemic brain. Our results also revealed a significant correlation between the impedance of the ischemic brain post-intervention and improvements in behavioral scores and infarct volume. This study shows that daily administration of transcranial focused ultrasound stimulation for 20 minutes to the ischemic hemisphere 24 hours after cerebral ischemia enhanced motor recovery in a rat model of ischemia. Additionally, our findings indicate that electrical impedance tomography can serve as a valuable tool for quantitatively evaluating rehabilitation after ischemic stroke in vivo . These findings suggest the feasibility of using impedance data collected via electrical impedance tomography to clinically assess the effects of rehabilitatory interventions for patients with ischemic stroke.

低强度经颅聚焦超声刺激对大鼠缺血性脑卒中的治疗作用:电阻抗断层成像的体内评价。
虽然已有研究表明经颅聚焦超声刺激对缺血脑有保护作用,但对刺激时间窗和强度缺乏明确的标准。电阻抗断层扫描能够实时监测缺血脑内脑血流灌注的变化,但研究使用这种方法评估脑卒中后体内康复的可行性仍然至关重要。本研究采用大脑中动脉闭塞术诱导大鼠缺血性卒中。采用经颅聚焦超声刺激治疗大鼠缺血模型,电阻抗断层扫描测量缺血急性期和刺激后康复期的阻抗。电阻抗成像结果显示缺血后脑阻抗升高,经颅聚焦超声刺激后脑阻抗降低。此外,刺激可促进大鼠缺血性脑卒中模型运动功能恢复,减少脑梗死体积,并诱导脑源性神经营养因子在缺血性脑中的表达。我们的研究结果还揭示了干预后缺血脑阻抗与行为评分和梗死体积改善之间的显著相关性。本研究表明,在脑缺血24小时后,每天对缺血半球进行20分钟的经颅聚焦超声刺激,可增强缺血大鼠模型的运动恢复。此外,我们的研究结果表明,电阻抗断层扫描可以作为定量评估体内缺血性中风后康复的有价值的工具。这些发现表明,使用电阻抗断层扫描收集的阻抗数据来临床评估缺血性卒中患者康复干预的效果是可行的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Neural Regeneration Research
Neural Regeneration Research CELL BIOLOGY-NEUROSCIENCES
CiteScore
8.00
自引率
9.80%
发文量
515
审稿时长
1.0 months
期刊介绍: Neural Regeneration Research (NRR) is the Open Access journal specializing in neural regeneration and indexed by SCI-E and PubMed. The journal is committed to publishing articles on basic pathobiology of injury, repair and protection to the nervous system, while considering preclinical and clinical trials targeted at improving traumatically injuried patients and patients with neurodegenerative diseases.
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