Highly Efficient Spectral Measurement Methods Using Newly Developed High-Throughput Magnetic Circularly Polarized Luminescence System

IF 2.8 4区 化学 Q2 CHEMISTRY, ANALYTICAL
Chirality Pub Date : 2024-12-12 DOI:10.1002/chir.70001
Satoko Suzuki, Akio Kaneta, Anas Santria, Taiji Oyama, Hiroyuki Nishikawa, Yoshitane Imai, Ken-ichi Akao, Naoto Ishikawa
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引用次数: 0

Abstract

Magnetic circularly polarized luminescence (MCPL) spectroscopy is widely used to evaluate the luminescence dissymmetry factor (gMCPL) for compounds. However, even for the same instrument and operating conditions, the measured gMCPL is affected by errors associated with sources such as baseline drift and spectral noise, and so the range of variation of gMCPL must be considered when comparing values, which requires multiple measurements for the same sample. Also, because many samples undergo photodegradation under excitation light, it is difficult to accumulate and average spectra for samples with weak MCPL signals to improve the signal-to-noise ratio. Single measurements must therefore be performed on multiple samples and the results averaged. Furthermore, for samples with a small Stokes shift, spectral correction is required to compensate for the intensity reduction due to the inner-filter effect (IFE). Such measurements are generally performed manually and are therefore time consuming and prone to human error. Here, we demonstrate the use of a newly developed high-throughput MCPL system to automatically measure MCPL and fluorescence spectra of multiple samples of phthalocyanine complexes with high efficiency and reduced human errors. This system allows the incorporation of effective countermeasures to the issues of gMCPL variation, sample photodegradation, extremely weak MCPL signals, and the IFE.

新开发的高通量磁圆偏振发光系统的高效光谱测量方法。
磁圆极化发光光谱(MCPL)被广泛用于评价化合物的发光不对称因子(gMCPL)。然而,即使在相同的仪器和操作条件下,测量的gMCPL也会受到与基线漂移和光谱噪声等源相关的误差的影响,因此在比较值时必须考虑gMCPL的变化范围,这需要对同一样品进行多次测量。此外,由于许多样品在激发光下发生光降解,因此难以对弱MCPL信号样品的光谱进行累积和平均,以提高信噪比。因此,必须对多个样品进行单次测量,并对结果取平均值。此外,对于Stokes位移较小的样品,需要进行光谱校正以补偿由于内滤波器效应(IFE)引起的强度降低。此类测量通常是手动执行的,因此非常耗时,而且容易出现人为错误。在这里,我们展示了使用新开发的高通量MCPL系统来自动测量多种酞菁配合物样品的MCPL和荧光光谱,效率高,减少了人为错误。该系统允许对gMCPL变化、样品光降解、极弱的MCPL信号和IFE等问题进行有效的对策。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Chirality
Chirality 医学-分析化学
CiteScore
4.40
自引率
5.00%
发文量
124
审稿时长
1 months
期刊介绍: The main aim of the journal is to publish original contributions of scientific work on the role of chirality in chemistry and biochemistry in respect to biological, chemical, materials, pharmacological, spectroscopic and physical properties. Papers on the chemistry (physiochemical, preparative synthetic, and analytical), physics, pharmacology, clinical pharmacology, toxicology, and other biological aspects of chiral molecules will be published.
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