Temperature-responsive regulation of the polycyclic aromatic hydrocarbon-degrading mesophilic bacterium Novosphingobium pentaromativorans US6-1 with a temperature adaptation system.

IF 3.9 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Applied and Environmental Microbiology Pub Date : 2024-12-12 DOI:10.1128/aem.01484-24

Zhuangzhuang Liu, Xinran Liu, Haiyan Huang, Feifei Cao, Qiu Meng, Tingheng Zhu, Jianhua Yin, Xiaofei Song, Zhiliang Yu

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引用次数: 0

Abstract

Survivability and tolerance of polycyclic aromatic hydrocarbon (PAH)-degrading bacteria in harsh environments, especially under varying temperatures, are a bottleneck for the effective application of in situ bioremediation. In this study, a temperature adaptation system (TAS) was constructed by combining a customized thermotolerant system with a customized cold-resistant system to realize the temperature-responsive regulation of the PAH-degrading mesophilic bacterium Novosphingobium pentaromativorans US6-1. The innovative dual-pronged TAS strategy enabled the chassis strain to effectively tackle conditions under varying temperatures, ensuring robust biological activities across a broadened temperature spectrum and exhibiting the potential to realize the high-efficiency PAH degradation of N. pentaromativorans US6-1 in in situ bioremediation. Furthermore, the temperature-responsive regulation achieved using the TAS circuit is likely promising for creating intelligent microbial cell factories and avoiding precise temperature maintenance, making it highly useful for industrial applications.IMPORTANCEEnvironmental temperature is among the extremely important factors that determine the bioactivities of pollutant-degrading microorganisms in in situ bioremediation. Effectively maintaining the survivability and tolerance of mesophilic microorganisms under harsh conditions and varying temperatures remains a challenge in the application of pollutant bioremediation. This study, for the first time, developed a temperature adaptation system by combining a customized thermotolerant system with a customized cold-resistant system to realize the temperature-responsive regulation of the polycyclic aromatic hydrocarbon (PAH)-degrading mesophilic bacterium Novosphingobium pentaromativorans US6-1, thus diminishing the need for precise temperature control in PAH bioremediation.

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来源期刊

Applied and Environmental Microbiology 生物-生物工程与应用微生物

CiteScore

7.70

自引率

2.30%

发文量

730

审稿时长

1.9 months

期刊介绍： Applied and Environmental Microbiology (AEM) publishes papers that make significant contributions to (a) applied microbiology, including biotechnology, protein engineering, bioremediation, and food microbiology, (b) microbial ecology, including environmental, organismic, and genomic microbiology, and (c) interdisciplinary microbiology, including invertebrate microbiology, plant microbiology, aquatic microbiology, and geomicrobiology.