In vitro effect of vitaminB12 on embyro growth by induction of hypoxia in culture.

Abstract

In this study, effects of vitaminB₁₂ on embryonic development have been investigated by supplying vitaminB₁₂ on a hypoxia-induced embryo culture. 9.5-day-old embryos from Wistar albino adult pregnant rats were used in our experimental set up.10 μM and 100 μM vitaminB₁₂ were added to culture medium which is then exposed to in vitro hypoxia. Additionally, 11.5-day-old embryos and yolksacs were examined morphologically. Different vitaminB₁₂ doses are compared within experimental groups. It was found that both control and experimental groups in 11.5-day-old embryos are at same developmental stage. It was also determined that oxygen deficiency influenced embryonic development and yolk sac vascularity in hypoxia group, are lagging behind in all experimental groups (P < 0.05). However, the development of vitaminB₁₂ embryos were similar to control group under normoxic conditions (P > 0.05). It was also observed that development was compensated through supplement of vitaminB₁₂ to hypoxia group (P < 0.05). It was indicated that the development in H + 100 μM vitB₁₂ groups was quite close to control group. However, development of H + 10 μM vitB₁₂ embryos were in parallel with hypoxic group. Furthermore, H + 100 μM vitB₁₂ group showed higher embryonic development than H + 10 μM vitB₁₂ group (P < 0.05).VitaminB₁₂ treatment has been used to prevent intrauterine growth restriction which can be caused by many different pharmacological agents. However, nobody has investigated effects of vitaminB₁₂ on hypoxia-induced early embryo growth retardation. In the light of our findings, administration of 100 μM vitaminB₁₂ restores damage of embryonic development due to hypoxia and this application also increases embryonic vascularity and circulation. Thus, supplementation of vitaminB₁₂ can be offered as a therapeutic approach towards cell death and diseases such as neurovascular and cardiovascular diseases and in the near future.