Assay for detection of adenosine phosphorylase from mycoplasmas

Abstract

A microtechnique is described which permits simple evaluation of the activity of the enzyme adenosine phoshorylase (AdoP), present in all mycoplasmas tested thus far. The good solubility and stability of AdoP and the sensitivity of the assay should be advantageous in detecting mycoplasmas in biological samples such as animal sera, cell cultures and vaccines.