3D Printed GelMA/CHIMA Cross-Linked Network Hydrogel for Angiogenesis

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology and Bioengineering Pub Date : 2024-12-11 DOI:10.1002/bit.28907

Zixian Liu, Meng Li, Rong Cheng, Lijing Wang, Peiyi Hou, Lu Han, Shengbo Sang

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Abstract

Vascularization is a key issue facing the construction of functional three-dimensional (3D) tissues, which is critical for the long-term survival and stability of tissue construct transplantation. In this study, a photocurable hydrogel material carboxymethyl chitosan (CHIMA) was successfully prepared and integrated with methacryloyl gelatin (GelMA) to construct the bioink GelMA/CHIMA, which was subsequently used 3D printing technology to prepared a bioactive scaffold with angiogenesis-inducing functionality. The results showed that the cross-linked GelMA/CHIMA bioink had a porous structure that supported cell growth and metabolism. The incorporation of CHIMA could significantly improve the hydrophilicity, swelling rate, pressure resistance and mechanical strength of the bioink. GelMA/CHIMA bioink supported the survival and continued proliferation of human umbilical vein endothelial cells (HUVECs) in the scaffold. In particular, the bioink composed of 8 wt% GelMA and 2 wt% CHIMA could stimulate the expression of angiogenesis genes. 3D printed bioactive scaffolds supported the survival of HUVECs and had abundant protein deposition including CD31 and VEGF. Therefore, this study constructed a bioactive scaffold with angiogenesis induction function, which provides a feasible strategy for the construction of vascularized complex tissues.

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3D打印凝胶/中国交联网络水凝胶血管生成

血管化是构建功能三维组织所面临的关键问题，对组织构建移植的长期存活和稳定性至关重要。本研究成功制备了一种可光固化的水凝胶材料羧甲基壳聚糖（CHIMA），并与甲基丙烯酰明胶（GelMA）结合构建了生物链接凝胶/CHIMA，随后利用3D打印技术制备了具有诱导血管生成功能的生物活性支架。结果表明，交联的GelMA/ china生物链具有支持细胞生长和代谢的多孔结构。掺入china可显著提高生物链的亲水性、溶胀率、耐压性和机械强度。GelMA/ china bioink支持支架中人脐静脉内皮细胞（HUVECs）的存活和持续增殖。其中，由8 wt% GelMA和2 wt% china组成的生物链接可以刺激血管生成基因的表达。3D打印的生物活性支架支持HUVECs的存活，并且具有丰富的CD31和VEGF蛋白沉积。因此，本研究构建了具有血管生成诱导功能的生物活性支架，为构建血管化复杂组织提供了一种可行的策略。

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来源期刊

Biotechnology and Bioengineering 工程技术-生物工程与应用微生物

CiteScore

7.90

自引率

5.30%

发文量

280

审稿时长

2.1 months

期刊介绍： Biotechnology & Bioengineering publishes Perspectives, Articles, Reviews, Mini-Reviews, and Communications to the Editor that embrace all aspects of biotechnology. These include: -Enzyme systems and their applications, including enzyme reactors, purification, and applied aspects of protein engineering -Animal-cell biotechnology, including media development -Applied aspects of cellular physiology, metabolism, and energetics -Biocatalysis and applied enzymology, including enzyme reactors, protein engineering, and nanobiotechnology -Biothermodynamics -Biofuels, including biomass and renewable resource engineering -Biomaterials, including delivery systems and materials for tissue engineering -Bioprocess engineering, including kinetics and modeling of biological systems, transport phenomena in bioreactors, bioreactor design, monitoring, and control -Biosensors and instrumentation -Computational and systems biology, including bioinformatics and genomic/proteomic studies -Environmental biotechnology, including biofilms, algal systems, and bioremediation -Metabolic and cellular engineering -Plant-cell biotechnology -Spectroscopic and other analytical techniques for biotechnological applications -Synthetic biology -Tissue engineering, stem-cell bioengineering, regenerative medicine, gene therapy and delivery systems The editors will consider papers for publication based on novelty, their immediate or future impact on biotechnological processes, and their contribution to the advancement of biochemical engineering science. Submission of papers dealing with routine aspects of bioprocessing, description of established equipment, and routine applications of established methodologies (e.g., control strategies, modeling, experimental methods) is discouraged. Theoretical papers will be judged based on the novelty of the approach and their potential impact, or on their novel capability to predict and elucidate experimental observations.

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