Transcriptome profiling of triploid Crassostrea gigas gills indicates the host immune mechanism against bacterial infection

IF 2.2 2区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2024-12-05 DOI:10.1016/j.cbd.2024.101392

Xiumei Liu , Weijun Wang , Haitao Zhao , Yongjie Wang , Liming Jiang , Enshuo Zhang , Yanwei Feng , Xumin Wang , Jiangyong Qu , Jianmin Yang , Zan Li

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Abstract

As an important member of global aquaculture, oysters (Crassostrea gigas) have significant economic value. With the development of commercial aquaculture, the frequent occurrence of diseases caused by Vibrio alginolyticus has become a hindrance to high-density aquaculture. Gill tissue, as an important component of immune system of the oysters, plays the key point in the face of invasion by foreign substances. Compared to the diploid oyster, the triploid oyster presents a higher growth rate and lower growth investment, making it a more ideal model for studying oyster immune defense. In this study, triploid oysters were as the research subject, and gill tissues attacked by V. alginolyticus were sequenced. By analyzing samples from different time points, 1746 DEGs were obtained. The KEGG and GO functional enrichment analysis showed that gill tissues mainly participate in immune function through the PIK3-Akt signaling pathway and the MAPK signaling pathway. The protein interaction network revealed three genes (CASP8, CASP9 and PIK3CA) that play core roles in immune defense by analyzing the interaction relationship between genes. Finally, qRT-PCR verified the expression of key genes. This study provides a more effective scientific basis for disease prevention and control of oysters and other bivalve shellfish, and helps to promote the sustainable development of aquaculture.

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三倍体长牡蛎鳃的转录组分析揭示了宿主对细菌感染的免疫机制。

牡蛎（Crassostrea gigas）作为全球水产养殖的重要成员，具有重要的经济价值。随着商业水产养殖的发展，溶藻弧菌引起的疾病频繁发生，已成为高密度养殖的阻碍。鳃组织作为牡蛎免疫系统的重要组成部分，在牡蛎抵御外来物质入侵时起着至关重要的作用。与二倍体牡蛎相比，三倍体牡蛎具有更高的生长速度和更低的生长投资，是研究牡蛎免疫防御的更理想的模型。本研究以三倍体牡蛎为研究对象，对受溶藻弧菌侵袭的牡蛎鳃组织进行了测序。通过分析不同时间点的样品，得到1746个deg。KEGG和GO功能富集分析表明，鳃组织主要通过PIK3-Akt信号通路和MAPK信号通路参与免疫功能。蛋白质相互作用网络通过分析基因间的相互作用关系，揭示了在免疫防御中发挥核心作用的三个基因（CASP8、CASP9和PIK3CA）。最后通过qRT-PCR验证关键基因的表达。本研究为牡蛎及其他双壳类贝类的疾病防治提供了更有效的科学依据，有助于促进水产养殖的可持续发展。

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来源期刊

Comparative Biochemistry and Physiology D-Genomics & Proteomics 生物-生化与分子生物学

CiteScore

5.10

自引率

3.30%

发文量

审稿时长

33 days

期刊介绍： Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology. Part D: Genomics and Proteomics (CBPD), focuses on “omics” approaches to physiology, including comparative and functional genomics, metagenomics, transcriptomics, proteomics, metabolomics, and lipidomics. Most studies employ “omics” and/or system biology to test specific hypotheses about molecular and biochemical mechanisms underlying physiological responses to the environment. We encourage papers that address fundamental questions in comparative physiology and biochemistry rather than studies with a focus that is purely technical, methodological or descriptive in nature.