Comparative transcriptome analysis and transient assays revealed AaGST and AaBGAL, respectively, contribute to skin and flesh coloration in A. arguta

IF 2.6 3区生物学 Q2 GENETICS & HEREDITY

Gene Pub Date : 2024-12-04 DOI:10.1016/j.gene.2024.149143

Xu Zhan , Yukuo Li , Zhe Song , Xiaohan Li , Lingshuai Ye , Miaomiao Lin , Ran Wang , Leiming Sun , Jinbao Fang , Dixin Chen , Xiujuan Qi

{"title":"Comparative transcriptome analysis and transient assays revealed AaGST and AaBGAL, respectively, contribute to skin and flesh coloration in A. arguta","authors":"Xu Zhan , Yukuo Li , Zhe Song , Xiaohan Li , Lingshuai Ye , Miaomiao Lin , Ran Wang , Leiming Sun , Jinbao Fang , Dixin Chen , Xiujuan Qi","doi":"10.1016/j.gene.2024.149143","DOIUrl":null,"url":null,"abstract":"<div><div><em>Actinidia arguta</em> possesses different colors in the fruit skin and flesh, but the underlying mechanism has not yet been clarified. In this study, we conducted 36 samples RNA-seq to investigate the phenotypic expression of different fruit tissues (skin and flesh) in red and green <em>A. arguta</em> varieties during different coloring phases. GO and KEGG enrichment results of differentially expressed genes (DEGs) suggested that the red color of the skin and flesh was derived from anthocyanin transport and flesh softening, respectively. Weighted gene co-expression network analysis (WGCNA) revealed MEyellow and MEblack modules significantly correlated with skin and flesh coloration, and two genes, Glutathione S-transferases (<em>AaGST</em>) and β-galactosidases (<em>AaBGAL)</em>, were identified as hub genes involved in different tissue-specific coloration. Transient overexpression in apples and kiwifruits confirmed the role of <em>AaGST</em> and <em>AaBGAL</em> in color formation. Our results preliminarily explore the mechanism of red color formation in different <em>A. arguta</em> fruit tissues and provide novel insights into red color formation.</div></div>","PeriodicalId":12499,"journal":{"name":"Gene","volume":"937 ","pages":"Article 149143"},"PeriodicalIF":2.6000,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0378111924010242","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}

引用次数: 0

Abstract

Actinidia arguta possesses different colors in the fruit skin and flesh, but the underlying mechanism has not yet been clarified. In this study, we conducted 36 samples RNA-seq to investigate the phenotypic expression of different fruit tissues (skin and flesh) in red and green A. arguta varieties during different coloring phases. GO and KEGG enrichment results of differentially expressed genes (DEGs) suggested that the red color of the skin and flesh was derived from anthocyanin transport and flesh softening, respectively. Weighted gene co-expression network analysis (WGCNA) revealed MEyellow and MEblack modules significantly correlated with skin and flesh coloration, and two genes, Glutathione S-transferases (AaGST) and β-galactosidases (AaBGAL), were identified as hub genes involved in different tissue-specific coloration. Transient overexpression in apples and kiwifruits confirmed the role of AaGST and AaBGAL in color formation. Our results preliminarily explore the mechanism of red color formation in different A. arguta fruit tissues and provide novel insights into red color formation.

Abstract Image

查看原文本刊更多论文

比较转录组分析和瞬时分析显示，AaGST和AaBGAL分别参与了软枣皮和肉的颜色。

软枣猕猴桃果皮和果肉具有不同的颜色，但其潜在的机制尚不清楚。本研究通过对36个样品的RNA-seq分析，研究了红、绿两种软枣品种在不同着色期不同果实组织（果皮和果肉）的表型表达。差异表达基因（DEGs）的GO和KEGG富集结果表明，皮肤和果肉的红色分别来源于花青素运输和果肉软化。加权基因共表达网络分析（WGCNA）显示MEyellow和MEblack模块与皮肤和肉的颜色显著相关，谷胱甘肽s -转移酶（AaGST）和β-半乳糖苷酶（AaBGAL）两个基因被确定为参与不同组织特异性颜色的中心基因。苹果和猕猴桃的瞬时过表达证实了AaGST和AaBGAL在颜色形成中的作用。本研究初步探讨了软枣果实不同组织红色形成的机制，为软枣果实红色形成提供了新的认识。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Gene 生物-遗传学

CiteScore

6.10

自引率

2.90%

发文量

718

审稿时长

42 days

期刊介绍： Gene publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses.