Production and characterization of monoclonal antibodies for specific detection of Nosema ceranae and Nosema apis in beehive samples.

IF 3.7 2区医学 Q1 PARASITOLOGY

International journal for parasitology Pub Date : 2024-12-03 DOI:10.1016/j.ijpara.2024.11.008

Fernando Izquierdo, Carmen Fernández Vadillo, Soledad Fenoy, Carolina Hurtado-Marcos, Angela Magnet, Mariano Higes, Raquel Martín-Hernández, Carmen Del Aguila

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引用次数: 0

Abstract

Two microsporidian species infect honeybees worldwide, Nosema apis and Nosema ceranae. Two different clinical patterns are considered: nosemosis type A (N. apis) and nosemosis type C (N. ceranae). Nosemosis type A is characterized in acute forms and nosemosis type C shows no clear outward clinical signs. The development of a rapid and simple tool for Nosema detection could allow beekeepers or veterinarians to carry out diagnostic tests in situ. Currently, PCR and microscopy are expensive techniques that require qualified staff and may not be available in every laboratory. The present study describes the production and characterization of four monoclonal antibodies (mAbs) against N. ceranae and N. apis, and the development of an IFAT. An IFAT using the mAbs was compared with microscopy and PCR for 180 beehive samples. The diagnostic test revealed similar sensitivity and specificity percentages to IFAT (97.79% and 93.18%, respectively) and microscopy (97.79% and 95.45%), considering 100% for the PCR as the 'gold standard'. A mAb (7D2) was patented for its high specificity for N. ceranae. The IFAT using the mAbs is a good alternative to microscopy and PCR in laboratories where PCR is not available for the detection and identification of both Nosema spp.

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来源期刊

International journal for parasitology 医学-寄生虫学

CiteScore

8.40

自引率

2.50%

发文量

审稿时长

23 days

期刊介绍： International Journal for Parasitology offers authors the option to sponsor nonsubscriber access to their articles on Elsevier electronic publishing platforms. For more information please view our Sponsored Articles page. The International Journal for Parasitology publishes the results of original research in all aspects of basic and applied parasitology, including all the fields covered by its Specialist Editors, and ranging from parasites and host-parasite relationships of intrinsic biological interest to those of social and economic importance in human and veterinary medicine and agriculture.