Optimization and Preparation of Doxycycline-Loaded Chitosan Nanoparticles Using Box-Behnken Design for Better Diabetic Wound Healing.

IF 3.7 3区 医学 Q2 CHEMISTRY, MEDICINAL
Harish Bhardwaj, Ram Kumar Sahu, Rajendra Kumar Jangde
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引用次数: 0

Abstract

A diabetic wound is one of the most devastating difficulties associated with diabetes and leads to significant death and morbidity. Hence, the aim was to make Doxycycline-loaded chitosan nanoparticles (DOX-CNPs) using ionic gelation with a cross-linking technique. In the Box-Behnken design, the DOX-CNPs were optimized by considering the effects of the following 3 variables independently, namely chitosan, sodium tripolyphosphate in volume ratio, strength of chitosan and sodium tripolyphosphate, among several response variables related to nanoparticle properties. The Fourier transform infrared, transmission electron microscopy, differential scanning calorimeter, X-ray diffraction, particle size, entrapment efficiency, and drug release in-vitro were used to characterized the nanoparticles. Additionally, DPPH scavenging activity and activity against Escherichia coli and Staphylococcus aureus bacteria and in vivo characterization were carried out to optimize DOX-CNPs. Then effective delivery of DOX-CNPs is incorporated in chitosan hydrogel for diabetic wounds. The findings of this study indicate that DOX-CNPs exhibit free radical scavenging properties, demonstrate significant antibacterial activity, and enhance cell viability and migration in an in vitro wound healing assay using the L929 fibroblast cell line, and in vivo demonstrate increased blood vessels, collagen deposition epithelization. Chitosan could be used as a drug carrier in a DOX-chitosan-NP system to help develop procedures that can be used in the lab and to treat diabetic wounds.

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来源期刊
CiteScore
7.30
自引率
13.20%
发文量
367
审稿时长
33 days
期刊介绍: The Journal of Pharmaceutical Sciences will publish original research papers, original research notes, invited topical reviews (including Minireviews), and editorial commentary and news. The area of focus shall be concepts in basic pharmaceutical science and such topics as chemical processing of pharmaceuticals, including crystallization, lyophilization, chemical stability of drugs, pharmacokinetics, biopharmaceutics, pharmacodynamics, pro-drug developments, metabolic disposition of bioactive agents, dosage form design, protein-peptide chemistry and biotechnology specifically as these relate to pharmaceutical technology, and targeted drug delivery.
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